DmeEX0006974 @ dm6
Exon Skipping
Gene
FBgn0040068 | Vav
Description
The gene Vav guanine nucleotide exchange factor is referred to in FlyBase by the symbol DmelVav (CG7893, FBgn0040068). It is a protein_coding_gene from Dmel. It has 5 annotated transcripts and 5 polypeptides (3 unique). Gene sequence location is X:19261820..19273280. Its molecular function is described by: Ras guanyl-nucleotide exchange factor activity; Rac guanyl-nucleotide exchange factor activity. It is involved in the biological process described with 20 unique terms, many of which group under: embryo development ending in birth or egg hatching; regulation of adherens junction organization; epithelial cell migration; protein phosphorylation; positive regulation of catalytic activity. 17 alleles are reported. The phenotypes of these alleles manifest in: eye-antennal disc; embryonic/larval dorsal branch; midline crossing tract; longitudinal connective; dorsal closure embryo. The phenotypic classes of alleles include: increased mortality during development; some die during pupal stage; phenotype; immune response defective. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages.
Coordinates
chrX:19263881-19269651:+
Coord C1 exon
chrX:19263881-19263955
Coord A exon
chrX:19264685-19265306
Coord C2 exon
chrX:19269484-19269651
Length
622 bp
Sequences
Splice sites
3' ss Seq
ATATGGGGAGACTCCCCCAGCGA
3' ss Score
-4.4
5' ss Seq
GAGGTGTGC
5' ss Score
5.35
Exon sequences
Seq C1 exon
TGGCTTCAACCTACAGCTTTCGCCCACGGAGCGTTCCCATTCGGACGAGGCCATCTACAAGGACCTGCATTCCAC
Seq A exon
CGATCTGAACATGCGGAAGACGAGCAGCATAGACGCCTCCGCCTCCTCGTCGGCGGAATACTACGACCGAACGTCGCAGAGCGGATCGCTGGACGAGAATAGCGATATTACGATCGAGAACGGCACCGAGGACATCGACGACTACATCGAGGACTCGCTCTCCAATATGTGCGACTCGACCATCGACAACGATGCGGAGGATGCGGGCGTCGAGACGCCCCTGCTTTCCGACTGCCAGGGCAGCCATGTGCGCGAGCTATCCTTCGATCTCGCCCTGGATGTGGTTAGCTCCACGACGGATGGCGCATCCAATGCGGTCACACCCACGCCGGAATCGTATGCCCGCCAATCGCAATCACCGTTGGCCGCCGCAGCGCCAAGCGTTCCTGTGTCCGCTGCACCGGGGCCACCTATGGGCCGTCTGGTGTCCACCTCCTCCTCATCGTCGTCGCTTCTGGTCAGCGAGAGCCAGCGGCTGCAGCGAGCGGCCGCCGCCGTCTACAACTACCGCTCCTCGATGGCGTCCACCGAGCACGAGTATGCCTACATCTACAGCGAGGACGACGAGAAGGTCTACGAGGATTTGTGCTACGTTACCTTCCAGGCGAAGGCCAAACCCGAG
Seq C2 exon
ACCGCCTCGTCGACGAGCTTTGAGCAGCGCGACTATGTCATCCGCGAGCTGATCGACACGGAGTCCAATTACCTGGACGTTCTCACTGCGCTGAAGACCAAGTTTATGGGTCCGCTGGAGCGGCATCTCAACCAGGACGAGCTGCGTCTCATATTCCCGCGCATCAGA
VastDB Features
Vast-tools module Information
Secondary ID
FBgn0040068_MULTIEX1-3/5=2-5
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.135 A=0.484 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0062115=RhoGEF=PU(24.4=76.8)
Main Inclusion Isoform:
FBpp0305901
Main Skipping Isoform:
FBpp0074448
Other Inclusion Isoforms:
FBpp0112228
Other Skipping Isoforms:
FBpp0074449
Associated events
Conservation
Human
(hg38)
No conservation detected
Human
(hg19)
No conservation detected
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Zebrafish
(danRer10)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTCAACCTACAGCTTTCGCCC
R:
CTGATGCGCGGGAATATGAGA
Band lengths:
238-860
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Neural diversity
- Neurogenesis
- Neuronal activity
- Splicing factor regulation (brain)
- Splicing factor regulation (SL2)