DreALTA0003921-2/2 @ danRer10
Alternative 3'ss
Gene
ENSDARG00000071090 | actn2b
Description
actinin, alpha 2b [Source:ZFIN;Acc:ZDB-GENE-051127-39]
Coordinates
chr17:20105637-20106850:-
Coord C1 exon
chr17:20106704-20106850
Coord A exon
chr17:20105703-20105767
Coord C2 exon
chr17:20105637-20105702
Length
65 bp
Sequences
Splice sites
5' ss Seq
CGGGTGCTG
5' ss Score
0.58
3' ss Seq
TGTGTATGTTTTTGTGTTAGGTG
3' ss Score
9.44
Exon sequences
Seq C1 exon
CATGTTCGCGTTGGCTGGGAGCTCCTATTGACCACGATTGCTCGCACAATTAATGAAATCGAGACTCAAATTTTGACCCGTGATGCAAAAGGGATCAGCCAGGAGCAGATGCATGAGTTCCGGTCCTCTTTCAACCATTTTGATCGG
Seq A exon
GTGCTGTCTCTTTCTCTACTATTATTCCTACTCTTGTCTTCTACCCTTTAATATTTGCCATGCAG
Seq C2 exon
AAGAAGAACGGGGGCATGGATTCTGATGACTTTAGGGCCTGTCTGATCTCGATGGGTTATGATCTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000071090-23-26,23-25-2/2
Average complexity
Alt3
Mappability confidence:
NA
Protein Impact
ORF disruption when splice site is used (sequence inclusion)
No structure available
Features
Disorder rate (disopred):
C1=0.014 A=NA C2=0.061
Domain overlap (PFAM):
C1:
PF0043516=Spectrin=PD(20.2=42.9),PF088696=XisI=PD(31.8=42.9),PF134051=EF-hand_6=PU(33.3=20.4)
A:
NA
C2:
PF138331=EF-hand_8=PU(37.7=90.9)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Cow
(bosTau6)
No conservation detected
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGCTGGGAGCTCCTATTGA
R:
GTCATCAGAATCCATGCCCCC
Band lengths:
167-232
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]