DreEX0018239 @ danRer10
Exon Skipping
Gene
ENSDARG00000009738 | cacnb4a
Description
calcium channel, voltage-dependent, beta 4a subunit [Source:ZFIN;Acc:ZDB-GENE-080320-2]
Coordinates
chr9:38310962-38314601:+
Coord C1 exon
chr9:38310962-38311020
Coord A exon
chr9:38311111-38311220
Coord C2 exon
chr9:38314450-38314601
Length
110 bp
Sequences
Splice sites
3' ss Seq
TTTTTTTACCTCTTTTTCAGAAT
3' ss Score
11.15
5' ss Seq
TGGGTATGA
5' ss Score
5.85
Exon sequences
Seq C1 exon
GTGACAGATATGATGCAGAAAGCCCTCTTTGACTTTCTGAAACACAGGTTTGAAGGCAG
Seq A exon
AATATCAATCACTAGAGTAACTGCAGACATCTCTTTAGCCAAGAGATCTGTCCTGAATAACCCTAGTAAGAGAGCCATCATTGAGAGATCCAACACCAGATCCAACCTGG
Seq C2 exon
CGGAGGTTCAGAGTGAGATCGAGCGGATCTTTGAATTGGCCAGATCCCTTCAGCTGGTTGTTCTCGATGCCGACACGATCAACCATCCTGCCCAGCTCATCAAGACTTCTTTAGCTCCCATTATTGTCCATGTTAAAGTGTCTTCTCCCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000009738_CASSETTE1
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.184 C2=0.000
Domain overlap (PFAM):
C1:
PF0062516=Guanylate_kin=FE(10.5=100)
A:
PF0062516=Guanylate_kin=FE(20.4=100)
C2:
PF0062516=Guanylate_kin=FE(27.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACAGATATGATGCAGAAAGCCC
R:
CTTGGGAGAAGACACTTTAACATGGA
Band lengths:
210-320
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]