DreEX0020499 @ danRer10
Exon Skipping
Gene
ENSDARG00000044811 | cdk9
Description
cyclin-dependent kinase 9 (CDC2-related kinase) [Source:ZFIN;Acc:ZDB-GENE-030131-321]
Coordinates
chr21:6036227-6042160:+
Coord C1 exon
chr21:6036227-6036317
Coord A exon
chr21:6040389-6040555
Coord C2 exon
chr21:6041989-6042160
Length
167 bp
Sequences
Splice sites
3' ss Seq
GCTGGTCTCATTTCTTTCAGCCA
3' ss Score
6.8
5' ss Seq
AAGGTACAA
5' ss Score
6.82
Exon sequences
Seq C1 exon
TTTCCCATCACGGCTCTGAGGGAGATCAAGATCTTGCAGCTTCTCAAACACGAGAATGTGGTCAACCTCATCGAGATCTGCAGAACAAAGG
Seq A exon
CCACCCAGTTTAACCGTTACAAGGGCAGCATTTATCTGGTGTTTGACTTCTGTGAGCACGACCTCGCTGGACTGCTGAGCAACGCTAATGTTAAGTTCACTCTGGCGGAGATCAAGAGGGTCATGCAGATGCTGCTCAACGGACTCTACTACATACACAGAAACAAG
Seq C2 exon
ATCCTCCACAGAGACATGAAAGCAGCCAATGTCCTGATCACCAGAGATGGTGTCCTGAAGCTGGCTGATTTCGGTCTGGCCCGAGCCTTCAGTTTGGCTAAAAACAGTCAGGGGAATCGCTACACAAATCGGGTGGTCACTCTGTGGTATAGACCGCCTGAACTACTGCTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000044811_MULTIEX1-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=FE(10.1=100)
A:
PF0006920=Pkinase=FE(18.5=100)
C2:
PF0006920=Pkinase=FE(19.1=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGCTCTGAGGGAGATCAAGA
R:
AGCAGTAGTTCAGGCGGTCTA
Band lengths:
251-418
Functional annotations
There are 1 annotated functions for this event
PMID: 23140174
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: alanine scanning, protein kinase assay, western blot. ELM ID: ELMI003213; ELM sequence: TKASPYNR; Overlap: PARTIAL_LEFT
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]