DreEX0021732 @ danRer10
Exon Skipping
Gene
ENSDARG00000086647 | chrng
Description
cholinergic receptor, nicotinic, gamma [Source:ZFIN;Acc:ZDB-GENE-030131-3805]
Coordinates
chr2:45238866-45243568:+
Coord C1 exon
chr2:45238866-45238980
Coord A exon
chr2:45241426-45241672
Coord C2 exon
chr2:45243435-45243568
Length
247 bp
Sequences
Splice sites
3' ss Seq
TGTATTTTATGATTGCTCAGGTG
3' ss Score
6.41
5' ss Seq
TACGTCAGT
5' ss Score
3.13
Exon sequences
Seq C1 exon
GTATCTGATGTTTGTGATGTCGGTGACCACAATAACTGTGATGAACTGTGTGGTGGTGTTAAACGTCTCTCTGAGAACTCCAAACACTCACCCCATGAGCAACACCATCAGAAAG
Seq A exon
GTGTTGTTGAACATCCTTCCTCGAGTGCTGAGGATGACGATGCAGCGCTGGACACCTGAGCAGGAGGAAGGGGATTTTAAGATGTTCGCTCTGGGCAATGGAACTCCGCTCCGCCGCAGGAGACGCAGCTCTTTAGGGTTAATAGCAAAGGCGGATGAATACATGTTCAGAACGGCACGATCCGAGCTCATGTTCAGCCGACTGAAGGAGAGGAAGGGGCTGCTGAAGAACACTCTGGAGAAAATAC
Seq C2 exon
AGAATGGTCTGGGGGGAAACACGGCACAGGATCTGGAGTGCAGTTTAGCAGCGGCCTCCCCTGAAGTGCAGCAGTGTGTGTCCTCCTGCAAACACATCGCAGAAAGCACCAAGCATCAAAATGACTTCCAAAGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000086647-'13-14,'13-13,14-14
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.044
Domain overlap (PFAM):
C1:
PF0293211=Neur_chan_memb=FE(14.8=100)
A:
PF0293211=Neur_chan_memb=FE(31.9=100)
C2:
PF0293211=Neur_chan_memb=FE(17.1=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGATGTTTGTGATGTCGGTGACC
R:
ACTTTGGAAGTCATTTTGATGCTTGG
Band lengths:
244-491
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]