DreEX0024163 @ danRer10
Exon Skipping
Gene
ENSDARG00000060081 | crb2b
Description
crumbs family member 2b [Source:ZFIN;Acc:ZDB-GENE-060610-1]
Coordinates
chr8:2804307-2812292:+
Coord C1 exon
chr8:2804307-2804502
Coord A exon
chr8:2805483-2805622
Coord C2 exon
chr8:2812110-2812292
Length
140 bp
Sequences
Splice sites
3' ss Seq
GCGCAGTGTGTGTTCGTCAGGTA
3' ss Score
5.02
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
Exon sequences
Seq C1 exon
GTCTCAACTGTGAAGTGGACGTGGATGAATGTGAAGCGTCTCCCTGCCAGAACGGCGCCGTCTGCCATGATCACGTGGGTCTGTACACCTGTGAGTGTGTGCCGGGGTACGAGGGCATCAACTGTGAGCTGGACATCGATGAGTGTGAGAGCTCACCCTGCCGCCATGGAGGAGTCTGTCTGGATATGGTCAACGG
Seq A exon
GTATGAGTGTGACTGCAGTGGCACTGGATTCATGGGCTTTTTCTGTGATATTGACATCCCCGAGTGTTCTTCTGACCCCTGTCAACATGGCGCCACCTGCCTGGAGGGTGTAAACCACTACACCTGCATCTGCTGGACAG
Seq C2 exon
GTTATGAAGGAGTGAACTGTGAGCTGGATGTGGACGAGTGTGTTTCTCAGCCATGTGAGAATGAAGGCCAGTGTTTCCAGCGCTCAGACTCCAGCCTCTACAGAGTTCTGCCTGAACTGGACACTGACTTCAGCTTCCAGAGCGCAGCCGGTTACCTGTGCCAGTGCGTATCTGGCTTCACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060081-'4-4,'4-3,5-4
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0000822=EGF=PD(6.5=3.0),PF0000822=EGF=WD(100=47.0),PF0000822=EGF=PU(58.6=25.8)
A:
PF0000822=EGF=PD(37.9=22.9),PF0000822=EGF=PU(80.6=52.1)
C2:
PF0000822=EGF=PD(16.1=8.1),PF0000822=EGF=PU(94.1=77.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATCACGTGGGTCTGTACACCT
R:
TCAGTGTCCAGTTCAGGCAGA
Band lengths:
254-394
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]