DreEX0025515 @ danRer10
Exon Skipping
Gene
ENSDARG00000053530 | cyp4f3
Description
cytochrome P450, family 4, subfamily F, polypeptide 3 [Source:ZFIN;Acc:ZDB-GENE-070410-108]
Coordinates
chr12:48570704-48572201:-
Coord C1 exon
chr12:48572068-48572201
Coord A exon
chr12:48570897-48570961
Coord C2 exon
chr12:48570704-48570786
Length
65 bp
Sequences
Splice sites
3' ss Seq
GTGTGTGTGTGTGTGTACAGGAT
3' ss Score
9.14
5' ss Seq
CTGGTAAGT
5' ss Score
10.65
Exon sequences
Seq C1 exon
GGAGGATCTGAGTCAGCTGACCTTCACCACCATGTGCATCAAGGAGAGTCTGAGGCTTCATTCACCCGTCCTGGCGCTCACCCGATACTACTCTCAGAACATGAAGACGCCAGGAGACTGCGTTATCCCACACG
Seq A exon
GATGCCTGTGTTTGATCAGTATTTATGGTGTCCACCGAAACCCTCAAGTCTGGCCGGATCCACTG
Seq C2 exon
GTGTTCGACCCGACGCGCTTCGACCCACATAACTCAGACAGCCGCTCTCCTCACGCCTTCATCCCGTTCTCCGCAGGTCCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000053530-'12-12,'12-11,13-12
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.143
Domain overlap (PFAM):
C1:
PF0006717=p450=FE(9.5=100)
A:
PF0006717=p450=FE(4.4=100)
C2:
PF0006717=p450=FE(5.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal4)
No conservation detected
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGAGTCAGCTGACCTTCACCA
R:
CGGCTGTCTGAGTTATGTGGG
Band lengths:
170-235
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]