DreEX0042455 @ danRer10
Exon Skipping
Gene
ENSDARG00000101209 | lamb1a
Description
laminin, beta 1a [Source:ZFIN;Acc:ZDB-GENE-021226-1]
Coordinates
chr25:31189932-31199929:-
Coord C1 exon
chr25:31199727-31199929
Coord A exon
chr25:31193554-31193674
Coord C2 exon
chr25:31189932-31190120
Length
121 bp
Sequences
Splice sites
3' ss Seq
TGTGTCTTCTTTTGTTGTAGGTT
3' ss Score
12.02
5' ss Seq
AAAGTAGGA
5' ss Score
3.63
Exon sequences
Seq C1 exon
ACATGTTGAAGATCACTAATCTGAGGGTGAAGTTCACTAAACTGCACACTCTCGGCGACAACCTGCTGGACTCACGTATAGAGATCAAGGAGAAGTATTACTATGCTATTTACGACATGGTGGTCAGAGGAAACTGCTTTTGCTATGGTCACGCATCTGAATGCGCCCCTGTCGATGGAACTGGAGAGGCAGTGGAGGGCATG
Seq A exon
GTTCATGGTCATTGTATGTGTAATCATAACACTATCGGTCTGAACTGTGAACGCTGTCAGGACTTTTACCATGATCTGCCCTGGAGACCCGCTGAGGGACGCAACACCAATGCTTGTAAAA
Seq C2 exon
AGTGTCACTGCAACCATCACTCTCATTCGTGCCATTTTGACATGGCAGTTTACCGAGCCTCAGGGAACGTGAGCGGCGGCGTGTGCGATGACTGCCAGCACAACACTATGGGCCACAACTGCGAACAGTGCAAACCCTTCTTCCACCAGCATCCAGAGAAGGACATCCGCGACCCCAACATCTGCGAGC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000101209-'8-9,'8-8,9-9
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0005512=Laminin_N=PD(18.2=63.2),PF0005319=Laminin_EGF=PU(35.5=32.4)
A:
PF0005319=Laminin_EGF=PD(61.3=92.7)
C2:
PF0005319=Laminin_EGF=WD(100=95.3)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGACATGGTGGTCAGAGGAA
R:
TGTCCTTCTCTGGATGCTGGT
Band lengths:
257-378
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]