DreEX0042507 @ danRer10
Exon Skipping
Gene
ENSDARG00000036279 | lamc1
Description
laminin, gamma 1 [Source:ZFIN;Acc:ZDB-GENE-021226-3]
Coordinates
chr2:35964168-35972186:+
Coord C1 exon
chr2:35964168-35964298
Coord A exon
chr2:35968639-35968805
Coord C2 exon
chr2:35971998-35972186
Length
167 bp
Sequences
Splice sites
3' ss Seq
CTCTGTTACCTTCCCTCCAGGTG
3' ss Score
12.15
5' ss Seq
TGCGTAAGT
5' ss Score
9.73
Exon sequences
Seq C1 exon
GATTGGGTAACTGCCACTGATATCAGAGTGACCCTGAATCGATTAAACACTTTCGGAGATGAAGTTTTCAACGATCCCAAAGTCCTCAAATCCTACTACTACGCCATCTCGGATTTTGCTGTGGGAGGAAG
Seq A exon
GTGCAAGTGCAATGGCCATGCCAGCGAGTGCGTGAAGAACGAGTACAGCAAGCTGGTGTGTAACTGCAAACACAACACAGAAGGAGCCGACTGCAATGTGTGCAAACCCTTCTATAATGACCGTCCCTGGAGGAGAGCCACTGCTGAAAACCCTAATGAGTGTCTGC
Seq C2 exon
CTTGTAACTGTAATGGAAAGAGCGCTGAATGTTACTTCGACCCTGAGCTGTATCGTGCCACTGGTCACGGAGGTCACTGCCGTAACTGTGCTGACAATACAGATGGGCCGAAGTGTGAGCGATGCCTGGCCAACTACTACAGAGAGGCCTCCGGTCAGCGCTGTCTGTCCTGCGGCTGCAACCCTGTTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000036279-'6-7,'6-6,8-7
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0005512=Laminin_N=PD(17.9=95.5)
A:
PF0005319=Laminin_EGF=WD(100=94.7)
C2:
PF0005319=Laminin_EGF=WD(100=84.4),PF0005319=Laminin_EGF=PU(13.3=9.4)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGAGTGACCCTGAATCGAT
R:
TCTGTAGTAGTTGGCCAGGCA
Band lengths:
252-419
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]