DreEX0050001 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23019654-23021568:-
Coord C1 exon
chr9:23021464-23021568
Coord A exon
chr9:23020943-23021032
Coord C2 exon
chr9:23019654-23019767
Length
90 bp
Sequences
Splice sites
3' ss Seq
CATTTCACTGCGCTTTTTAGGTG
3' ss Score
8.31
5' ss Seq
GATGTAAGA
5' ss Score
5.46
Exon sequences
Seq C1 exon
ATTGGATACCGTGATAAGGCTCGTCAGGAGGCCAGCCGTGGTGGAACACTGCTCGGCCGTCCTGACATCGCCCTGGCCACTGAGGTGTCCAAACTGACCAGTCAG
Seq A exon
GTGGAGGAAAAACCATCCCTCCATGGTGCTGCCTCCTTTGACACTCCTCAGATGCGGCATATTAAGAAAATGAGTGCCCTGACTAGTGAT
Seq C2 exon
GTAAAGTACAAGGAGAAGTTTGACAAGGAAATGAAAGGAAAGAGACCACAGTATGACTTGAAGAACAGCAAGATATATCAGACTCTGAAGGATGCTAATACACTTGCAAGCGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-1/13=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.490 A=0.667 C2=0.305
Domain overlap (PFAM):
C1:
PF112763=DUF3078=PD(14.7=31.4),PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
PF0088013=Nebulin=PU(55.2=53.3)
C2:
PF0088013=Nebulin=PD(57.1=30.8),PF0088013=Nebulin=PU(56.0=35.9)
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm10)
No conservation detected
Mouse
(mm9)
No conservation detected
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGTGATAAGGCTCGTCAGGA
R:
TGCTGTTCTTCAAGTCATACTGTGG
Band lengths:
167-257
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]