DreEX0050012 @ danRer10
Exon Skipping
Gene
ENSDARG00000032630 | neb
Description
nebulin [Source:ZFIN;Acc:ZDB-GENE-041111-216]
Coordinates
chr9:23000931-23021568:-
Coord C1 exon
chr9:23021464-23021568
Coord A exon
chr9:23011116-23011229
Coord C2 exon
chr9:23000931-23001038
Length
114 bp
Sequences
Splice sites
3' ss Seq
TGTCTGACCACTTTCTGCAGAGA
3' ss Score
6.71
5' ss Seq
CAGGTACAC
5' ss Score
7.29
Exon sequences
Seq C1 exon
ATTGGATACCGTGATAAGGCTCGTCAGGAGGCCAGCCGTGGTGGAACACTGCTCGGCCGTCCTGACATCGCCCTGGCCACTGAGGTGTCCAAACTGACCAGTCAG
Seq A exon
AGAGAGTACAAGAAAGCTTTGGAGCAAGAGATCAAGGGAAAAGGCATGTTGGCTTTGGCTACTGACACTCCAGACTTCTTGAGGGCAAGGAACGCCACAGAAATCCTCAGTCAG
Seq C2 exon
GTTTTGTACTCTGACAGCTTCCGTAAGCAGGTGCAAGGAAAGGCCGCCTTTGTGCTGGACACCCCCGAGATGAGACGCGTCAAAGAAACCCAGCGTATCATTTCTGGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032630_MULTIEX1-8/13=C1-C2
Average complexity
C3
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.490 A=0.077 C2=0.106
Domain overlap (PFAM):
C1:
PF112763=DUF3078=PD(14.7=31.4),PF0088013=Nebulin=PD(37.9=31.4),PF0088013=Nebulin=PU(55.2=45.7)
A:
PF0088013=Nebulin=PD(41.4=30.8),PF0088013=Nebulin=PU(55.2=41.0)
C2:
PF0088013=Nebulin=PD(37.9=30.6),PF0088013=Nebulin=PU(55.2=44.4)
Main Skipping Isoform:
ENSDART00000143888fB4191
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGGATACCGTGATAAGGCTCGT
R:
TCCAGAAATGATACGCTGGGT
Band lengths:
211-325
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]