DreEX0056143 @ danRer10
Exon Skipping
Gene
ENSDARG00000062590 | pleca
Description
plectin a [Source:ZFIN;Acc:ZDB-GENE-030131-8984]
Coordinates
chr19:22823985-22837996:-
Coord C1 exon
chr19:22837953-22837996
Coord A exon
chr19:22833679-22835168
Coord C2 exon
chr19:22823985-22832362
Length
1490 bp
Sequences
Splice sites
3' ss Seq
AAAGGCTGAAGAAGAGGCAGAGA
3' ss Score
-21.61
5' ss Seq
GAGGTATTG
5' ss Score
7.44
Exon sequences
Seq C1 exon
CGCTTTATCATGATGAATGTTTGTCATGTTTGGGTGCTAAAGAG
Seq A exon
AGAAATTTAGAAAACTTGCTTTGGAAGAAGAGAAAAAAAGGAAAGAGGCAGAGGCTAAGGTGAAACAAATCCAAGCTGCAGAGGAAGAGGCTGCAAGGCAACACAAAGCTGCCCAAGAGGAGGTTGGTCGTCTTATGAAACTAGCAGAAGAGGCTAAAAAGCAGAAGGAGATTGCAGAGAAGGAAGCTGAGAAACAGGTCATTTTAGTCCAGGAGGCTGCACAGAAATGTTCTGCGGCAGAGCAGAAAGCACAAAATGTTCTTGTCCAGCAAAATAAAGACAGCATGGCACAGGATAAACTCAAGGAAGAGTTTGAGAAAGCAAAGAAACTGGCGCAAGAAGCAGAAAAAGCTAAAGACAATGCAGAAAAAGAAGCTGCTCTTCTGCATAAGAAAGCAGAGGAAGCTGAGCGCCAAAAGAAGGCAGCTGAGGCAGAAGCAGCTAAACAGGCCAAAGCCCAGGAAGATGCTGAAAAACTGAGGAAAGAGGCTGAAAAAGAAGCTTCTAGGCGAGCTGAGGCAGAGGCTGCTGCTTTGAAACTAAAACAAGAGGCTGATAGTGAAATGGCCAAGTACAAAAAACTTGCAGAAAAGACCCTGAAGCAGAAGTCTTCAGTTGAGGAGGAGTTGGTGAAGGTTAAAGTTCAACTTGATGAAACAGACAAACAAAAATCAGTCCTTGATGTTGAACTTAAGCGACTTAAACAAGAGGTCAGTGATGCTATAAAGCAAAAGGCTCAAGTAGAAGATGAGCTCTCTAAAGTCAAGATTCAAATGGAGGACTTACTTAAACTAAAACTCAAAATTGAAAAGGAAAACCAAGAACTTATGAAAAAAGATAAAGATAACACAAAGAAACTTCTTGAGGAAGAGGCTGAGAACATGAAAAAATTGGCAGAAGAGGCTGCACGGCTCAACATTGAGGCACAAGAAGCTGCGAGATTGAGACAAATTGCAGAGTCTGATCTCGCCAAGCAGAGGGAACTGGCCGAGAAGATGTTGGAAGAGAAAAAACAGGCTATTCAAGAGGCAGCAAAACTGAAGGCTGAAGCTGAAAAGCTCCAGAAACAGAAAGATCAGGCACAGGTAGAAGCTCAAAAGCTGTTGGAGGCCAAGAAAGAGATGCAGCAGCGTCTGGACCAGGAGACCGAAGGTTTCCAGAAATCCTTAGAAGCTGAGCGAAAACGGCAATTGGAGATTACTGCAGAAGCTGAGAAACTAAAGGTCAAGGTGACACAGCTAAGTGATGCTCAGTCAAAAGCAGAGGAGGAAGCCAAAAAATTCAAGAAACAAGCTGATGAAATCAAAATCCGCCTGCAGGAGACTGAAAAGCACACTTCAGAAAAACACACTGTTGTTGAGAAACTGGAAGTACAGAGGCTACAGAGCAAACAAGAGGCTGACGGTTTGCACAAAGCAATTGCAGACCTTGAGAAAGAAAAGGAAAAACTGAAAAAAGAGGCCGCTGACTTACAAAAACAATCCAAAGAG
Seq C2 exon
ATGGCCAATGTACAACAAGAACAGTTACAACAAGAAAAGACGATCCTTCAGCAGAGTTTCTTTGCAGAAAAAGAGACACTGCTGAAGAAGGAGAAAGCTATTGAGGAAGAGAAAAAGAAGCTGGAGAAACAGTTTGAAGATGAAGTCAAGAAGGCTGAAGCACTTAAAGCTGAACAGGAGCGTCAGAGAAAGCTAATGGAGGAGGAGAGAAAGAAACTGCAGTCTGCCATGGATGCTGCTATTAAAAAGCAAAAGGAAGCTGAGGAAGAGATGAATGGCAAGCAAAAAGAAATGCAAGATCTTGAAAAGAAGAGAATTGAACAAGAGAAACTTCTGGCAGAAGAGAACAAGAATTTGCGGGAAAAACTCCAACAGCTTCAGAGTTCACAGAAGGCATCTTACACCAAGGAGATTGAAATCCAAACTGACAAGGTCCCCGAGGAAGAGTTAGTTCAAATGACCATGGTAGAAACAACAAAGAAGGTCCTTAATGGCTCTAC
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062590_MULTIEX1-14/14=13-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.679 C2=0.146
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=1.7),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=1.8),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=1.8),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=1.8),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=1.7),PF0068115=Plectin=WD(100=1.5),PF0068115=Plectin=WD(100=2.1),PF0068115=Plectin=WD(100=2.1)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGTTTGTCATGTTTGGGTGCT
R:
TCTCTTCTTTTCAAGATCTTGCATT
Band lengths:
342-1832
Functional annotations
There are 1 annotated functions for this event
PMID: 33986153
SNRPA1 enhances metastatic lung colonization and cancer cell invasion, in part through SNRPA1-mediated regulation of PLEC alternative splicing, which can be counteracted by splicing modulating morpholinos.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]