DreEX0060845 @ danRer10
Exon Skipping
Gene
ENSDARG00000079291 | rapgef3
Description
Rap guanine nucleotide exchange factor (GEF) 3 [Source:ZFIN;Acc:ZDB-GENE-090312-38]
Coordinates
chr23:33510740-33514436:+
Coord C1 exon
chr23:33510740-33510804
Coord A exon
chr23:33510901-33510955
Coord C2 exon
chr23:33514365-33514436
Length
55 bp
Sequences
Splice sites
3' ss Seq
CTTTTTTTATCTTGTTTTAGCGC
3' ss Score
10.78
5' ss Seq
ACGGTACGG
5' ss Score
9.91
Exon sequences
Seq C1 exon
ATATGACATTTATTCATGAGGGAAACAAGAACTACACTGATAAACTGGTCAACTTTGAGAAAATG
Seq A exon
CGCATGATTGCCAGAACAGTGAAGACAGTTCGGGATTGTCGAAGCCAACCTTACG
Seq C2 exon
TGCCTTCATCTCCGCAGAAAGGCTTGACGGAGAGAATGTTCTTGGATGCTCAAGCTATCCGAATATCAACAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079291-'28-29,'28-28,29-29
Average complexity
C1
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.263 C2=0.120
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(11.3=100)
A:
PF0061714=RasGEF=PD(0.5=5.3)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TGACATTTATTCATGAGGGAAACAA
R:
TGTTGATATTCGGATAGCTTGAGCA
Band lengths:
133-188
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]