DreEX0060875 @ danRer10
Exon Skipping
Gene
ENSDARG00000074307 | rapgef5b
Description
Rap guanine nucleotide exchange factor (GEF) 5b [Source:ZFIN;Acc:ZDB-GENE-061215-2]
Coordinates
chr16:19339960-19342429:+
Coord C1 exon
chr16:19339960-19340047
Coord A exon
chr16:19341526-19341590
Coord C2 exon
chr16:19342375-19342429
Length
65 bp
Sequences
Splice sites
3' ss Seq
AGCATATTTTATCTGAACAGATA
3' ss Score
6.68
5' ss Seq
CTAGTGAGT
5' ss Score
7.43
Exon sequences
Seq C1 exon
GACCCTTCGTTAAACCACAAAGCCTACAGAGATGCCTTCAGGAAGATGAAGTCTCCCAAGATCCCTTTCCTACCTCTGCTTCTGAAAG
Seq A exon
ATATTACCTTTATTCATGAAGGCAACAAAACCTTTCTCGACAACCTGGTCAACTTCGACAAGCTA
Seq C2 exon
CACATGATAGCAGACACAGTACGAATTATCAGGCAGTGTCGGACAGATCATATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000074307_MULTIEX1-1/2=C1-2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(15.7=100)
A:
PF0061714=RasGEF=FE(11.4=100)
C2:
PF0061714=RasGEF=PD(0.5=5.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GACCCTTCGTTAAACCACAAAGC
R:
CCATATGATCTGTCCGACACTGC
Band lengths:
143-208
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]