DreEX0065082 @ danRer10
Exon Skipping
Gene
ENSDARG00000062352 | sema4ab
Description
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4Ab [Source:ZFIN;Acc:ZDB-GENE-060503-363]
Coordinates
chr19:24798597-24800848:+
Coord C1 exon
chr19:24798597-24798712
Coord A exon
chr19:24800441-24800604
Coord C2 exon
chr19:24800760-24800848
Length
164 bp
Sequences
Splice sites
3' ss Seq
TGTCTGTATGCGTCCTGCAGGGG
3' ss Score
8.13
5' ss Seq
GCTGTGAGT
5' ss Score
7.39
Exon sequences
Seq C1 exon
AGTCAGGCTTCCTGCACAAAACTGTGCTCTTGAAGAAGGGAGCTCACATCATCGAGGAGATACAAGTGTTTCATCAACCTCAGATGGTCAAAAGCCTCCTTCTGTCTGTTTCTAAG
Seq A exon
GGGGCAGTGCTGATCGGCTCTTCAGAAGGGCTGCTGTCAGTGCCAGTGTCCAACTGCTCTCACTACAGGAGCTGTGCTGAATGTGTTCTGGCCAGAGACCCTTTCTGTGGCTGGGACCTTTCCCAGAGGGTCTGCAAACTGATAACCACATCACAGAATCAGCT
Seq C2 exon
GGCTCAGGATGTAGATGAGGGTAAAGTATCAGAGCAGTGCTCCAGTGTGAACCTCAGATCCCGCTCTGGCCAGCCCCAAAACACCCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000062352_CASSETTE2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.131
Domain overlap (PFAM):
C1:
PF0140314=Sema=FE(9.1=100)
A:
PF0140314=Sema=PD(0.1=0.0),PF0143720=PSI=PU(54.4=66.1)
C2:
PF0143720=PSI=FE(44.1=100),PF138951=Ig_2=PU(6.5=19.4)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGGCTTCCTGCACAAAACTG
R:
TCTGGGTGTTTTGGGGCTGG
Band lengths:
202-366
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]