DreEX6007145 @ danRer10
Exon Skipping
Gene
ENSDARG00000015349 | mfge8a
Description
milk fat globule-EGF factor 8 protein a [Source:ZFIN;Acc:ZDB-GENE-050327-9]
Coordinates
chr7:48520402-48524463:-
Coord C1 exon
chr7:48524350-48524463
Coord A exon
chr7:48523922-48524103
Coord C2 exon
chr7:48520402-48520557
Length
182 bp
Sequences
Splice sites
3' ss Seq
ATCTTTTTTTTGTGCTGCAGGCT
3' ss Score
9.42
5' ss Seq
GAGGTTTGC
5' ss Score
3.52
Exon sequences
Seq C1 exon
ATGTGAATGACTGCGCAAACAAGCCTTGTATGAACGGAGGTGTGTGTCGTGATCTGGATGGCGACTACACCTGCCAGTGCCCTTCTCCATATGTCGGAAAACAGTGCAAGCTAC
Seq A exon
GCTGTACATCTCTATTGGGGATGGAGGAAGGAGGAATCATAGAGTCCCAGATTTCAGCTTCTTCTGTTCACTCTGGAATTCTGGGACTGCAGCACTGGGGACCTTCGCTTGCACGCCTTAATAACCGGGGGATTGTCAATGCCTGGAGCTCAGCACCCCAAGACAAGAACCCCTGGATTGAG
Seq C2 exon
ATAGACATGCAAAAGAAGATGCGTCTCACAGGAATCATCACTCAAGGTGCCAGTCGCATGGGGACAGCTGAGTTTATCAAGGCTTTCAAGGTGGCATCCAGTTTTGATGGCAAAACCTACACTGTGTACAGAGCAGATGGCCAGAGAAAAGACAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000015349-'5-5,'5-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0000822=EGF=WD(100=79.5)
A:
PF0075420=F5_F8_type_C=PU(31.7=72.1)
C2:
PF0075420=F5_F8_type_C=FE(36.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAATGACTGCGCAAACAAGCC
R:
TCTGGCCATCTGCTCTGTACA
Band lengths:
255-437
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]