DreEX6008937 @ danRer10
Exon Skipping
Gene
ENSDARG00000063126 | gusb
Description
glucuronidase, beta [Source:ZFIN;Acc:ZDB-GENE-070705-256]
Coordinates
chr5:1833970-1840923:-
Coord C1 exon
chr5:1840738-1840923
Coord A exon
chr5:1835307-1835488
Coord C2 exon
chr5:1833970-1834112
Length
182 bp
Sequences
Splice sites
3' ss Seq
TTTTGTTTCCCATCATGCAGTGG
3' ss Score
6.54
5' ss Seq
AAGGTCAGA
5' ss Score
6.8
Exon sequences
Seq C1 exon
ACGGGTCCAGTGATTGACATGCCGGTGCCGTCCAGCTACAATGACATCACACAAGACGCCAAAATCAGGGATTTTGTTGGCTGGGTTTGGTATGAGAAGGAAGTGCTGGTTCCCACACGCTGGATCCAGGACCGGGGAACACGCATCGTGCTCCGAGTGGGAAGTGCACACTATTACTCTGTGCTT
Seq A exon
TGGGTGAACGGGGTCCAGATGACTGAACATGCGGGCGGTCATCTGCCATTCGAGGCGGACATCAGCAGCGTTCTGCGTCAGAGCTCTGGAGGTCCGTGTCGAATCACCATAGCAGTGAACAACACTTTATCTCTGGAAACACTTCCACCAGGAACCATCCAGTACATGAACGACCGCACAAG
Seq C2 exon
ATATCCTTCTGGCTACTTCGTTCAGAACACTAACTTTGATTTCTTCAACTATGCTGGAATACATCGCTCCGTCCTGCTGTACACGACACCCGCGGTGCACATTGATGACATCACAGTCCAGACGACATTCTCCGATAACATCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000063126-'1-2,'1-1,3-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.016 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0283713=Glyco_hydro_2_N=FE(32.3=100)
A:
PF0283713=Glyco_hydro_2_N=FE(31.7=100)
C2:
PF0283713=Glyco_hydro_2_N=PD(15.9=61.2),PF0070316=Glyco_hydro_2=PU(16.2=32.7)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGGTCCAGTGATTGACATGCC
R:
GACGGAGCGATGTATTCCAGC
Band lengths:
257-439
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]