Special

MmuEX0022230 @ mm9

Exon Skipping

Gene
ENSMUSG00000025534 | Gusb
Description
glucuronidase, beta [Source:MGI Symbol;Acc:MGI:95872]
Coordinates
chr5:130475797-130476812:-
Coord C1 exon
chr5:130476627-130476812
Coord A exon
chr5:130476231-130476412
Coord C2 exon
chr5:130475797-130475939
Length
182 bp
Sequences
Splice sites
3' ss Seq
ATCTCTCCTTGTGTCTGCAGTGG
3' ss Score
8.71
5' ss Seq
CATGTGAGC
5' ss Score
5.03
Exon sequences
Seq C1 exon
TCGGGCCCAGTCTTGGACATGCCTGTCCCTTCTAGCTTCAATGACATCACCCAAGAAGCAGCCCTTCGGGACTTTATTGGCTGGGTGTGGTATGAACGGGAAGCAATCCTGCCACGGCGATGGACCCAAGATACCGACATGAGAGTGGTGTTGAGGATCAACAGTGCCCATTATTATGCAGTTGTG
Seq A exon
TGGGTGAATGGGATTCATGTGGTGGAACATGAGGGAGGTCACCTCCCCTTTGAGGCTGACATTAGCAAGCTGGTCCAGAGTGGGCCCCTGACCACCTGCCGGATCACGATTGCCATCAACAACACACTGACCCCTCATACCCTTCCGCCGGGGACCATCGTCTACAAGACTGACACCTCCAT
Seq C2 exon
GTATCCCAAGGGTTACTTTGTCCAGGACACAAGCTTTGACTTCTTCAACTATGCGGGACTGCATCGATCTGTGGTCCTCTATACCACCCCTACCACTTACATCGATGATATCACTGTGATCACTAATGTGGAGCAAGACATCG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000025534_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact





	

	
ORF disruption upon sequence exclusion





No structure available

		

			
Features


			

				
Disorder rate (Iupred):
  C1=0.000 A=0.000 C2=0.022
 
                        

				
Domain overlap (PFAM):

					





						

							
C1:
PF0283713=Glyco_hydro_2_N=FE(32.3=100)

							
A:
PF0283713=Glyco_hydro_2_N=FE(31.7=100)

							
C2:
PF0283713=Glyco_hydro_2_N=PD(15.9=61.2),PF0070316=Glyco_hydro_2=PU(16.2=32.7)

						

					

				








    
Main Inclusion Isoform:
ENSMUSP00000026613





     
                   









    
Main Skipping Isoform:
ENSMUSP00000106939





     
      









    
Other Inclusion Isoforms:
ENSMUSP00000026614, ENSMUSP00000106940
          









    
Other Skipping Isoforms:
NA
  



Associated events






Other assemblies


mm10




Conservation


Human
(hg38)
chr7:65979727-65979911 
ALTERNATIVE
HsaEX0029113
(GUSB)
Human
(hg19)
chr7:65444821-65444898 
ALTERNATIVE
HsaEX0029113
(GUSB)
Rat
(rn6)
chr12:30204580-30204761 
HIGH PSI
RnoEX0040719
(Gusb)
Cow
(bosTau6)
chr25:28172369-28172550 
HIGH PSI
BtaEX0016597
(GUSB)
Chicken
(galGal4)
chr19:4929539-4929720 
HIGH PSI
GgaEX6017211
(GUSB)
Chicken
(galGal3)
chr19:4895672-4895853 
HIGH PSI
GgaEX6017211
(GUSB)
Zebrafish
(danRer10)
chr5:1835307-1835488 
HIGH PSI
DreEX6008937
(gusb)
Fruitfly
(dm6)
No conservation detected




Primers PCR

	

		

			
Suggestions for RT-PCR validation

			

				
F: 
ACATGCCTGTCCCTTCTAGCT

				
R: 
AGAGGACCACAGATCGATGCA

				
Band lengths: 
250-432

			

		

	





	
Functional annotations
(Submit an annotation)

	


There are 0 annotated functions for this event 


		










GENOMIC CONTEXT[edit]












INCLUSION PATTERN[edit]






SPECIAL DATASETS
  • Pre-implantation embryo development
  • Neural differentiation time course
  • Muscular differentiation time course
  • Spermatogenesis cell types
  • Reprogramming of fibroblasts to iPSCs
  • Hematopoietic precursors and cell types






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