DreEX6011329 @ danRer10
Exon Skipping
Gene
ENSDARG00000035505 | nelfb
Description
negative elongation factor complex member B [Source:ZFIN;Acc:ZDB-GENE-040426-765]
Coordinates
chr5:28953537-28955261:-
Coord C1 exon
chr5:28955076-28955261
Coord A exon
chr5:28953736-28953848
Coord C2 exon
chr5:28953537-28953639
Length
113 bp
Sequences
Splice sites
3' ss Seq
TTCTGTTTGCTCTTTTGTAGTTT
3' ss Score
8.31
5' ss Seq
AGGGTATGA
5' ss Score
6.84
Exon sequences
Seq C1 exon
GTGGTGCTGAAGCTGACTCAGATGATTGGGAAGAACGTGAAGCTCTATGATATGGTGCTGCAGTTCTTGCGAACGCTGTTTCTGCGTACACGTAATGTACATTACTGCACCTTGCGAGCGGAGCTGCTCATGTCCCTTCATGACCTCGACATCAGCGAGATCTGTTCAGTCGACCCCTGTCACAAG
Seq A exon
TTTTCCTGGTGTTTGGATGCCTGCATCAGGGAGAAGTTTGTGGATGCTAAACGGGCCAGAGAGTTACAAGGCTTTTTGGATGGTGTGAAGAAAGGCCAGGAGCAAGTGTTAGG
Seq C2 exon
TGACCTTTCTATGATCCTCTGTGACCCCTTTGCCTGTAACACACTGGTGTTGAGCACTGTTAGGAACCTTCAGGAGTTGTTGAGTCAGGACGCCCTGCCCAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035505-'5-9,'5-8,6-9=AN
Average complexity
A_S
Mappability confidence:
88%=100=75%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(12.9=100)
A:
PF062098=COBRA1=FE(7.8=100)
C2:
PF062098=COBRA1=FE(7.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGGGAAGAACGTGAAGCTCT
R:
GCGTCCTGACTCAACAACTCC
Band lengths:
254-367
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]