MmuEX0012054 @ mm10
Exon Skipping
Gene
ENSMUSG00000013465 | Nelfb
Description
negative elongation factor complex member B [Source:MGI Symbol;Acc:MGI:1931035]
Coordinates
chr2:25203887-25206345:-
Coord C1 exon
chr2:25206160-25206345
Coord A exon
chr2:25205127-25205239
Coord C2 exon
chr2:25203887-25203982
Length
113 bp
Sequences
Splice sites
3' ss Seq
TGTGTATTTCCTTCCTGTAGTTC
3' ss Score
8.65
5' ss Seq
GGGGTGAGA
5' ss Score
4.74
Exon sequences
Seq C1 exon
GTGGTGCAGAAGCTGACGCAGATGGTAGGGAAGAATGTGAAGCTGTACGACATGGTGCTGCAGTTCCTGCGCACACTGTTCCTGCGGACCAGGAATGTGCACTACTGCACGTTACGAGCTGAGCTGCTCATGTCTTTGCATGACTTGGACGTCAGTGACATCTGCACTGTGGATCCCTGCCATAAG
Seq A exon
TTCACCTGGTGCCTGGATGCCTGCATCCGGGAACGGTTTGTGGACAGCAAAAGAGCCCGGGAGCTTCAGGGATTTCTTGATGGTGTGAAGAAGGGCCAGGAACAAGTTTTGGG
Seq C2 exon
GATAGTCCCGACCTCCTCCTACTACTTAGGCTGCTGGCACTGGGCCAAGGTGCATGGGACTTAATAGATAGCCAGGTCTTCAAGGAGCCTAAGATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000013465_MULTIEX2-1/2=C1-C2
Average complexity
C2
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF062098=COBRA1=PD(10.5=3.2),PF062098=COBRA1=FE(37.3=100)
A:
PF062098=COBRA1=FE(22.3=100)
C2:
PF062098=COBRA1=PU(7.6=91.7)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GAAGCTGACGCAGATGGTAGG
R:
TTAAGTCCCATGCACCTTGGC
Band lengths:
242-355
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types