DreEX6011331 @ danRer10
Exon Skipping
Gene
ENSDARG00000035505 | nelfb
Description
negative elongation factor complex member B [Source:ZFIN;Acc:ZDB-GENE-040426-765]
Coordinates
chr5:28950733-28951262:-
Coord C1 exon
chr5:28951174-28951262
Coord A exon
chr5:28950953-28951094
Coord C2 exon
chr5:28950733-28950841
Length
142 bp
Sequences
Splice sites
3' ss Seq
CTTTGTGTGTGTGTTTGCAGTGG
3' ss Score
8.95
5' ss Seq
AAGGTCTGC
5' ss Score
5.38
Exon sequences
Seq C1 exon
GGTGGCGTGTGAGATTGGCCTTTACTATGCTCTGCATATTGCCAAGCTGAGGAATAAAAATGCTTTACAGCGGCTTCTGCCTGCATTGG
Seq A exon
TGGATACATATAATGACATGGCATTCGGAGATATATTTCTCCATCTGCTCACTGGTCATCTGACTCTTCTCTCTGATGAGTTTGGAACAGAGGAGTTTTGTACTGCTGTCTTTGATAACTTCCTACTCACGTCTTTCTCAAG
Seq C2 exon
TAAGGAGAACGTTCACAGGCATTGCCTTCGCTTGCTGAATCATCTCCATCAGAAGGTGCTGCCGACTTACATGGAAACCTTGATGAAGACGCTTGAACCACCAAAACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000035505-'13-17,'13-16,14-17=AN
Average complexity
A_S
Mappability confidence:
88%=75=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.189
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(7.6=100)
A:
PF062098=COBRA1=FE(10.0=100)
C2:
PF062098=COBRA1=FE(7.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCGTGTGAGATTGGCCTTTA
R:
TGTTTTGGTGGTTCAAGCGTC
Band lengths:
194-336
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]