RnoEX0059301 @ rn6
Exon Skipping
Gene
ENSRNOG00000009377 | Nelfb
Description
negative elongation factor complex member B [Source:RGD Symbol;Acc:1307832]
Coordinates
chr3:2415375-2423280:-
Coord C1 exon
chr3:2423174-2423280
Coord A exon
chr3:2415754-2415895
Coord C2 exon
chr3:2415375-2415483
Length
142 bp
Sequences
Splice sites
3' ss Seq
GCCACCCTGTTTGTTTACAGTGG
3' ss Score
7.32
5' ss Seq
CAGGTAGGA
5' ss Score
9.79
Exon sequences
Seq C1 exon
GTTTCTGCAGGAGCAGCGCATGGCTTGTGAGGTGGGACTGTACTATGTTCTACACATCACCAAGCAGAGAAACAAGAATGCTCTCCTGCGCCTGCTGCCTGGTCTTG
Seq A exon
TGGAAACCTTTGGTGACCTGGCCTTCAGTGACATCTTCCTCCACCTACTCACTGGGAGTCTGGCGCTGTTGGCTGATGAATTTGCCCTGGAGGACTTTTGCAGCAGCCTCTTTGATGGCTTCTTCCTCACTGCCTCTCCCAG
Seq C2 exon
GAAGGACAATGTACACCGCCATGTGCTGAGGCTTCTCCTTCACTTGCATGCGAGAGTGGCCCCTTCCAAGCTGGAGGCGTTGCAGAAGGCGCTGGAGCCCACGGGCCAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000009377-'15-29,'15-26,18-29
Average complexity
S
Mappability confidence:
100%=100=80%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.081
Domain overlap (PFAM):
C1:
PF062098=COBRA1=FE(7.6=100)
A:
PF062098=COBRA1=FE(9.9=100)
C2:
PF062098=COBRA1=FE(7.6=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CATGGCTTGTGAGGTGGGAC
R:
CTCCAGCGCCTTCTGCAAC
Band lengths:
186-328
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]