DreEX6016247 @ danRer10
Exon Skipping
Gene
ENSDARG00000034588 | scn4ab
Description
sodium channel, voltage-gated, type IV, alpha, b [Source:ZFIN;Acc:ZDB-GENE-051201-1]
Coordinates
chr3:22142529-22143823:+
Coord C1 exon
chr3:22142529-22142844
Coord A exon
chr3:22142961-22143079
Coord C2 exon
chr3:22143734-22143823
Length
119 bp
Sequences
Splice sites
3' ss Seq
CCTTTTTTTCTGTTTCGCAGACG
3' ss Score
11.55
5' ss Seq
CTCATATCC
5' ss Score
-13.36
Exon sequences
Seq C1 exon
GAGAGACCCCGAAGTGGTGGCAGCCCTCGTCCAGCAGAATGTCAAGATGGCGCGTCTGCTCCCTCCCACAGGCACCGATGTGTTTCGGCCCCTCACTCTGGAGTCGCTCGCTGAGATTGATCGGCGGATGGCAGAGGAAGCCGCCGAGCAGGAGCGCATGAAGGAGCAGAATGTCAAGGTGGCCGAGGAGGATCTGCCCAAACCCACCAGTGATCTGGAAGCTGGGAAAGTCCTGCCGTTTATCTATGGAGACCCTCCACCAAATCTCCTCAATGTTCCTATTGAAGAGTTGGATCCGTACTACAAAGCACAAAAA
Seq A exon
ACGTTCATCGTTATAGACAAAAAGAACACGATATACAGATTCAACACTGAACCAGCCTGTTACTGTCTGAGTCCCTTCAACCCTGTTCGACGAGCTGCCATAAGAATACTCATACACTC
Seq C2 exon
ATTGTTTAGCTTAGTCATCATGCTAACCATCCTGACAAACTGTGTGTTCATGGCGATGAGCGACCCTCCAGGCTGGAGCAAAATATTAGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000034588-'0-1,'0-0,1-1=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.289 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF0052026=Ion_trans=PU(0.8=6.5)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Mouse
(mm9)
No conservation detected
Chicken
(galGal3)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTGAGATTGATCGGCGGATGG
R:
ACACACAGTTTGTCAGGATGGT
Band lengths:
253-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]