DreEX6017688 @ danRer10
Exon Skipping
Gene
ENSDARG00000060192 | rpusd1
Description
RNA pseudouridylate synthase domain containing 1 [Source:ZFIN;Acc:ZDB-GENE-060929-444]
Coordinates
chr3:35412559-35415110:+
Coord C1 exon
chr3:35412559-35412839
Coord A exon
chr3:35412940-35413133
Coord C2 exon
chr3:35414987-35415110
Length
194 bp
Sequences
Splice sites
3' ss Seq
TATATGTTGTGTTTGTGCAGGAG
3' ss Score
7.92
5' ss Seq
CAGGTAATT
5' ss Score
8.55
Exon sequences
Seq C1 exon
AGCATGGGACCAGTAATATAGCATTAAAGTAAATTTATTGAATCTACGCTGACACGTTGGAAAAAAAACTAACGTTCTTAAACAATTACTGAAGATGAACGTGTGCACGATCTGATGATTCCTCTAAAGGAGAAGGTTTGCTCTGCAGAGGGAACATATAAACAGAAGACTGGACGCGAGTGATCCACAGGCAACATGATTATCAACAACATTTAAGTCAGGATCCTTTTCGATACAAGTACAGCGGTAACACGCCAAGCAGCACGCGATAACCACGCCAG
Seq A exon
GAGAAGACGAGCATGGAGCCTGCGAGCCTGGAAAACCTGTGTGTTTTGTATCAAAGTACAAACTATATAGTTGTCAACAAACACTGGGACATTCGAATTGACAGTAAAATGTGGTATGAGAAGCAGACTGTCCAGAGTCAGCTAAAGCATCGCTTTCCAGAGCTTGCAGATCCTGGCACTTACTACGGCTTCAG
Seq C2 exon
GTTTTGCCATCAGTTGGATTTCTCCACCAGTGGTGCTTTGTGTGTTGCTCTGAATAAGGCGGCAGCAGGTCAAGCGTATCGCTGTTTTAAAGACAGACGGGTTACCAAAGCTTATCTAGCACTG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060192-'0-4,'0-0,1-4=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion (Ref, Alt. ATG (<=10 exons))
No structure available
Features
Disorder rate (Iupred):
C1=NA A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
NA
A:
PF0084917=PseudoU_synth_2=PU(26.5=72.1)
C2:
PF0084917=PseudoU_synth_2=FE(24.7=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTTTGCTCTGCAGAGGGAACA
R:
GCTTTGGTAACCCGTCTGTCT
Band lengths:
257-451
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]