DreEX6020120 @ danRer10
Exon Skipping
Gene
ENSDARG00000075924 | RAPGEF4 (1 of 2)
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:HGNC Symbol;Acc:HGNC:16626]
Coordinates
chr6:6745440-6746377:+
Coord C1 exon
chr6:6745440-6745500
Coord A exon
chr6:6746040-6746116
Coord C2 exon
chr6:6746231-6746377
Length
77 bp
Sequences
Splice sites
3' ss Seq
GTTTGCTCTCTGAATTTCAGTGT
3' ss Score
8.26
5' ss Seq
AAGGTAATG
5' ss Score
8.99
Exon sequences
Seq C1 exon
GTGAAGAGAGAGCTCGCCGGTGTCCTCATCTTTGAGTCACACGCCAAAGCAGGCACTGTCT
Seq A exon
TGTTTAATCAAGGGGAGGAAGGCACTTCTTGGTATATTATCCAGAAAGGATCAGTAAATGTTGTCATCTATGGCAAG
Seq C2 exon
GGTGTGGTTTGTACGCTGCATGAGGGAGATGATTTTGGCAAACTGGCGCTGGTAAATGACGCCCCCCGCGCCGCTTCCATCGTGCTGAGAGAGGACAACTGCCACTTCCTGAGGGTGGACAAAGAGGACTTCAACCGTATACTCAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075924-'13-15,'13-14,14-15=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=PU(22.5=42.9)
A:
PF0002724=cNMP_binding=FE(62.5=100)
C2:
PF0002724=cNMP_binding=FE(55.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGGTGTCCTCATCTTTGAGT
R:
CCTCTTTGTCCACCCTCAGGA
Band lengths:
172-249
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]