MmuEX6102152 @ mm9
Exon Skipping
Gene
ENSMUSG00000049044 | Rapgef4
Description
Rap guanine nucleotide exchange factor (GEF) 4 [Source:MGI Symbol;Acc:MGI:1917723]
Coordinates
chr2:72034273-72036962:+
Coord C1 exon
chr2:72034273-72034333
Coord A exon
chr2:72036394-72036470
Coord C2 exon
chr2:72036816-72036962
Length
77 bp
Sequences
Splice sites
3' ss Seq
CTCCATCTTTTTGTGTCTAGTGT
3' ss Score
6.95
5' ss Seq
AAGGTATAA
5' ss Score
7.08
Exon sequences
Seq C1 exon
GTGAAACGGGAGTTAGCAGGTGTTCTCATTTTTGAGTCTCACGCCAAAGGAGGAACTGTGT
Seq A exon
TGTTTAACCAGGGGGAAGAAGGTACCTCCTGGTACATCATTCTGAAAGGATCCGTGAATGTAGTCATTTATGGCAAG
Seq C2 exon
GGTGTGGTCTGCACCCTGCACGAAGGAGATGACTTTGGCAAGTTAGCTCTAGTGAACGATGCTCCAAGAGCTGCCTCCATTGTTCTTCGGGAAGATAATTGTCACTTCCTAAGAGTCGACAAGGAAGACTTCAATCGGATTCTGAGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000049044-'27-22,'27-21,28-22=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0002724=cNMP_binding=PU(10.3=42.9)
A:
PF0002724=cNMP_binding=FE(28.7=100)
C2:
PF0002724=cNMP_binding=FE(55.2=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTGAGTCTCACGCCAAAGGAG
R:
CCGATTGAAGTCTTCCTTGTCGA
Band lengths:
168-245
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
Other AS DBs: