DreEX6021215 @ danRer10
Exon Skipping
Gene
ENSDARG00000020187 | GCA
Description
grancalcin, EF-hand calcium binding protein [Source:HGNC Symbol;Acc:HGNC:15990]
Coordinates
chr6:13866118-13874914:-
Coord C1 exon
chr6:13874871-13874914
Coord A exon
chr6:13869765-13869912
Coord C2 exon
chr6:13866118-13866231
Length
148 bp
Sequences
Splice sites
3' ss Seq
CTTTAACCATGTATGTTCAGAGA
3' ss Score
3.66
5' ss Seq
TGGGTAAGT
5' ss Score
10.24
Exon sequences
Seq C1 exon
CTTTCAGTTTGGAAACCTGCAGGATTATGATTGCCTTGTTGGAT
Seq A exon
AGAGATTACACAGGAAAGATGGGATTCAATGAGTTTAAAGAGCTTTTTGGTGTGCTGAATGGCTGGAAGCAGAACTTCATGATGGTGGACAGGGACCACAGTGGGACCGTGGAACCTTACGAGATGTCCCAGTCCATTGCTAACATGG
Seq C2 exon
GGTATCGGGTTAGCCCACGGGTATTGGACGCCATTGTCAAACGCTACAGCAGGTCAGGAAAGATCTACTTTGATGACTATGTGGCGTGTTGTGTGAAGCTAAAAGCACTTACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000020187-'4-5,'4-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF138331=EF-hand_8=FE(24.6=100)
A:
PF138331=EF-hand_8=PD(29.8=34.0),PF134991=EF-hand_7=PU(48.3=58.0)
C2:
PF134991=EF-hand_7=PD(50.0=76.9)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCAGTTTGGAAACCTGCAGGA
R:
TGTAAGTGCTTTTAGCTTCACACA
Band lengths:
154-302
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]