DreEX6029056 @ danRer10
Exon Skipping
Gene
ENSDARG00000014674 | acsl3b
Description
acyl-CoA synthetase long-chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]
Coordinates
chr2:47642403-47644094:+
Coord C1 exon
chr2:47642403-47642580
Coord A exon
chr2:47643764-47643873
Coord C2 exon
chr2:47643956-47644094
Length
110 bp
Sequences
Splice sites
3' ss Seq
ATGATCTGAATGGTTTTCAGTGG
3' ss Score
5.46
5' ss Seq
AAGGTAAAA
5' ss Score
8.38
Exon sequences
Seq C1 exon
GTGATTTTGGGCGATTATAACTGGTTGTCGTATCAAGACACTTTCCATCTGGCTCAGAGGTTTGGAAGTGGACTGGCTGCGCTGGGTCAGAAGCCTCTCTGCAACATCGCTATTTTTTGCGAGACTCGAGCTGAATGGGTCATAGCGGCACAGGCTTGCTTCATGTACAATTTCCCCT
Seq A exon
TGGTGACTCTTTACTCTACACTGGGAGGATCAGCTATAGCTCATGGTCTGAACGAGACTGAGGTCACACACATCGTCACAAGCAAAGACCTGCTGCAGAGCCGCCTCAAG
Seq C2 exon
GCTATTCTGTTAGAAGTCCCCAGACTCAAGCACATTATAGTGGTCGACACGAAACCCACAAGCTGGCCTGATCTCCCCAGAGGCATCATGGTACATAACATGGCAGCGGTGCAAGAATTGGGATCCAAACCTGAAAACA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014674-'11-10,'11-9,12-10=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
PF0050123=AMP-binding=FE(7.5=100)
C2:
PF0050123=AMP-binding=FE(9.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGCTCAGAGGTTTGGAAGTGG
R:
TGGATCCCAATTCTTGCACCG
Band lengths:
255-365
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]