MmuEX6025666 @ mm10
Exon Skipping
Gene
ENSMUSG00000032883 | Acsl3
Description
acyl-CoA synthetase long-chain family member 3 [Source:MGI Symbol;Acc:MGI:1921455]
Coordinates
chr1:78688237-78692220:+
Coord C1 exon
chr1:78688237-78688414
Coord A exon
chr1:78690269-78690378
Coord C2 exon
chr1:78692082-78692220
Length
110 bp
Sequences
Splice sites
3' ss Seq
CTGTATTCTTTTCCTTGTAGTTG
3' ss Score
8.54
5' ss Seq
AAGGTGAGG
5' ss Score
9.16
Exon sequences
Seq C1 exon
GTTATTCTGGGGCACTATAATTGGCTTTCCTATGAAGATGTCTTCATCCGAGCCCTTGACTTTGGAAATGGGTTACAAATGTTGGGCCAGAAACCAAAGGCCAACATCGCCATCTTCTGTGAGACCAGGGCTGAGTGGATGATCGCTGCACAGGCGTGTTTTATGTATAACTTCCAGC
Seq A exon
TTGTTACACTGTATGCTACTCTGGGAGGTCCAGCCATTGTTCATGGACTGAATGAGACAGAGGTGACCAACATCATTACTAGCAAAGAACTCTTGCAAACAAAGCTGAAG
Seq C2 exon
GATATAGTCTCTTTGGTCCCACGTCTGCGGCATATCATTACTGTTGATGGGAAGCCTCCAACCTGGTCTGAGTTCCCCAAAGGTGTCATTGTACACACCATGGCTGCAGTGCAGGCTCTAGGAGTGAAGGCCAACGTGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032883-'21-18,'21-17,23-18
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
PF0050123=AMP-binding=FE(7.6=100)
C2:
PF0050123=AMP-binding=FE(10.6=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCTTCATCCGAGCCCTTGACT
R:
CTGCAGCCATGGTGTGTACAA
Band lengths:
247-357
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types