BtaEX6011841 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000017258 | ACSL3
Description
acyl-CoA synthetase long-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:3570]
Coordinates
chr2:111866440-111868876:+
Coord C1 exon
chr2:111866440-111866617
Coord A exon
chr2:111867777-111867886
Coord C2 exon
chr2:111868738-111868876
Length
110 bp
Sequences
Splice sites
3' ss Seq
GCCCTTTCTTTTCCCTGTAGTTG
3' ss Score
9.93
5' ss Seq
AAGGTGAGA
5' ss Score
8.68
Exon sequences
Seq C1 exon
GTTATTCTTGGAAGCTATAATTGGCTTTCCTACGAAGATGTCTTTGTGAGAGCCTTTAATTTTGGAAACGGCTTACAGATGCTGGGTCAGAAGCCCAAGACCAACATCGCCATCTTCTGTGAGACCAGGGCAGAGTGGATGGTTGCTGCACAGGCCTGCTTCATGTATAATTTTCAGC
Seq A exon
TTGTTACGCTGTATGCTACTCTAGGGGGTCCAGCAATTGTTCATGGATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTGCAGACAAAATTGAAG
Seq C2 exon
GACATCGTTTCTCTGGTCCCTCGCCTGCGGCACATCATCACTGTAGATGGAAAGCCGCCGACCTGGTCTGAGTTCCCCAAGGGCGTCATCGTGCACACCATGGCGGCCGTGCGGGCTCTGGGAGCCAAGGCCAGCATGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000017258-'13-9,'13-8,14-9=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
PF0050123=AMP-binding=FE(7.6=100)
C2:
PF0050123=AMP-binding=FE(9.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AAACGGCTTACAGATGCTGGG
R:
CATGCTGGCCTTGGCTCC
Band lengths:
251-361
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]