BtaEX6011840 @ bosTau6
Exon Skipping
Gene
ENSBTAG00000017258 | ACSL3
Description
acyl-CoA synthetase long-chain family member 3 [Source:HGNC Symbol;Acc:HGNC:3570]
Coordinates
chr2:111867777-111870629:+
Coord C1 exon
chr2:111867777-111867886
Coord A exon
chr2:111868738-111868876
Coord C2 exon
chr2:111870479-111870629
Length
139 bp
Sequences
Splice sites
3' ss Seq
GGATCCTTGTGTGTTTCCAGGAC
3' ss Score
8.31
5' ss Seq
TGGGTACGT
5' ss Score
7.7
Exon sequences
Seq C1 exon
TTGTTACGCTGTATGCTACTCTAGGGGGTCCAGCAATTGTTCATGGATTAAATGAAACAGAGGTGACCAACATCATTACTAGTAAAGAACTCTTGCAGACAAAATTGAAG
Seq A exon
GACATCGTTTCTCTGGTCCCTCGCCTGCGGCACATCATCACTGTAGATGGAAAGCCGCCGACCTGGTCTGAGTTCCCCAAGGGCGTCATCGTGCACACCATGGCGGCCGTGCGGGCTCTGGGAGCCAAGGCCAGCATGG
Seq C2 exon
AAAACAAACCCCTTAGCAAACCAGCGCCCTCAGATATTGCAGTAATCATGTACACGAGTGGATCCACAGGAATTCCAAAGGGGGTCATGATCTCTCATAGTAACATTATTGCTGGTATAACTGGGATGGCGGAAAGGATTCCAGAACTCGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSBTAG00000017258-'14-11,'14-9,15-11=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.020
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.6=100)
A:
PF0050123=AMP-binding=FE(9.7=100)
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACGCTGTATGCTACTCTAGGGG
R:
GAATCCTTTCCGCCATCCCAG
Band lengths:
246-385
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]