RnoEX0006168 @ rn6
Exon Skipping
Gene
ENSRNOG00000014718 | Acsl3
Description
acyl-CoA synthetase long-chain family member 3 [Source:RGD Symbol;Acc:70552]
Coordinates
chr9:84576925-84580791:+
Coord C1 exon
chr9:84576925-84577034
Coord A exon
chr9:84578569-84578707
Coord C2 exon
chr9:84580641-84580791
Length
139 bp
Sequences
Splice sites
3' ss Seq
ATTGTTATGTGTTTCTTTAGGAT
3' ss Score
10.61
5' ss Seq
TGGGTATGT
5' ss Score
7.43
Exon sequences
Seq C1 exon
TTGTTACACTGTATGCGACTCTGGGAGGTCCAGCCATTGTCCATGGACTGAATGAGACAGAGGTGACCAACATCATTACTAGTAAAGAACTCCTGCAAACAAAGCTGAAG
Seq A exon
GATATCGTCTCTTTGGTCCCACGTCTGCGGCATATCATTACTGTTGATGGGAAGCCTCCAACCTGGTCTGAGTTCCCCAAGGGCGTCATTGTACACACCATGGCTGCAGTGCAGGCTCTAGGAGTAAAGGCTGACGTGG
Seq C2 exon
ACAAGAAAGCTCACAGCAAACCACTGCCCTCAGATATTGCAGTAATCATGTACACAAGTGGATCCACAGGAATTCCAAAGGGAGTCATGATCTCACACAGCAACATCATTGCCTCTATAACGGGGATGGCGAGAAGGATTCCAAGACTGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSRNOG00000014718-'4-6,'4-4,6-6
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.6=100)
A:
PF0050123=AMP-binding=FE(9.7=100)
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Human
(hg19)
No conservation detected
Chicken
(galGal4)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACACTGTATGCGACTCTGGGA
R:
TTGGAATCCTTCTCGCCATCC
Band lengths:
249-388
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]