DreEX0010824 @ danRer10
Exon Skipping
Gene
ENSDARG00000014674 | acsl3b
Description
acyl-CoA synthetase long-chain family member 3b [Source:ZFIN;Acc:ZDB-GENE-030131-492]
Coordinates
chr2:47643764-47647788:+
Coord C1 exon
chr2:47643764-47643873
Coord A exon
chr2:47643956-47644094
Coord C2 exon
chr2:47647638-47647788
Length
139 bp
Sequences
Splice sites
3' ss Seq
CATGATCCTCCATCTCTCAGGCT
3' ss Score
8.33
5' ss Seq
ACAGTAAGC
5' ss Score
5.94
Exon sequences
Seq C1 exon
TGGTGACTCTTTACTCTACACTGGGAGGATCAGCTATAGCTCATGGTCTGAACGAGACTGAGGTCACACACATCGTCACAAGCAAAGACCTGCTGCAGAGCCGCCTCAAG
Seq A exon
GCTATTCTGTTAGAAGTCCCCAGACTCAAGCACATTATAGTGGTCGACACGAAACCCACAAGCTGGCCTGATCTCCCCAGAGGCATCATGGTACATAACATGGCAGCGGTGCAAGAATTGGGATCCAAACCTGAAAACA
Seq C2 exon
TAATGGTGTCCCGCAGGCAGCCTGTCCCATCTGATATCGCAGTGATCATGTACACCAGCGGCTCTACAGGCATTCCTAAAGGAGTCATGATCTCTCACAGCAACATCATCGCGGGCATCACTGGCATGGCTGAACGCATCCCGAACCTTGA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000014674-'12-12,'12-10,13-12
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.020
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.5=100)
A:
PF0050123=AMP-binding=FE(9.6=100)
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTCTACACTGGGAGGATCAGC
R:
CAAGGTTCGGGATGCGTTCAG
Band lengths:
248-387
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]