MmuEX6025665 @ mm10
Exon Skipping
Gene
ENSMUSG00000032883 | Acsl3
Description
acyl-CoA synthetase long-chain family member 3 [Source:MGI Symbol;Acc:MGI:1921455]
Coordinates
chr1:78690269-78694346:+
Coord C1 exon
chr1:78690269-78690378
Coord A exon
chr1:78692082-78692216
Coord C2 exon
chr1:78694196-78694346
Length
135 bp
Sequences
Splice sites
3' ss Seq
TGATTGTTGTGTTTCTTCAGGAT
3' ss Score
10.38
5' ss Seq
AACGTGGGT
5' ss Score
4.57
Exon sequences
Seq C1 exon
TTGTTACACTGTATGCTACTCTGGGAGGTCCAGCCATTGTTCATGGACTGAATGAGACAGAGGTGACCAACATCATTACTAGCAAAGAACTCTTGCAAACAAAGCTGAAG
Seq A exon
GATATAGTCTCTTTGGTCCCACGTCTGCGGCATATCATTACTGTTGATGGGAAGCCTCCAACCTGGTCTGAGTTCCCCAAAGGTGTCATTGTACACACCATGGCTGCAGTGCAGGCTCTAGGAGTGAAGGCCAAC
Seq C2 exon
AAAAGAAAGCTCACAGCAAACCACTGCCCTCAGATATTGCAGTAATCATGTACACAAGTGGGTCCACAGGAATTCCAAAGGGAGTCATGATCTCACACAGCAACATCATTGCTTCTATAACGGGGATGGCGAGAAGGATTCCAAGACTGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000032883-'23-19,'23-18,24-19
Average complexity
S*
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.6=100)
A:
PF0050123=AMP-binding=FE(10.6=100)
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTATGCTACTCTGGGAGGTCCA
R:
TTGGAATCCTTCTCGCCATCC
Band lengths:
244-379
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Ribosome-engaged transcriptomes of neuronal types
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types