DreEX6078216 @ danRer10
Exon Skipping
Gene
ENSDARG00000032079 | acsl3a
Description
acyl-CoA synthetase long-chain family member 3a [Source:ZFIN;Acc:ZDB-GENE-050420-181]
Coordinates
chr15:43095538-43100399:+
Coord C1 exon
chr15:43095538-43095647
Coord A exon
chr15:43098308-43098446
Coord C2 exon
chr15:43100249-43100399
Length
139 bp
Sequences
Splice sites
3' ss Seq
GTCTGTCTTTTGACCCACAGAGC
3' ss Score
9.64
5' ss Seq
ATGGTAATA
5' ss Score
5.05
Exon sequences
Seq C1 exon
TGGTAACACTTTACTCTACTCTGGGTGGTCCTGCTATTGCTCATGGGCTCAATGAAGCCGAAGTTTCTCACATCATCACCAGCAAGGATCTTCTGGAAACCAAACTCAAG
Seq A exon
AGCATTTTCATCGAGGTGCCCAGATTGTGCCACGTCATCGTGGTTGACGATAAGCTCAGCACCAGCTCTGAATACCCACGAGGCATCAGCATCCACAGCATGGCGTCGGTGAAAGAACTTGGGGCTCGTCCTGAGAATG
Seq C2 exon
CGTCAAAGCCGAAAAATCCTCCATGCTCCTCTGATGTGGCTGTGATCATGTACACCAGCGGATCCACTGGAGTGCCGAAAGGAGTCATGATCTCTCACAGTAACATCATCTCTGGGATCACCGGAATGACACACAGGATTCCCAAGCTCGT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032079-'6-6,'6-5,7-6=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.128 C2=0.137
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(7.6=100)
A:
PF0050123=AMP-binding=FE(9.7=100)
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ACTCTGGGTGGTCCTGCTATT
R:
CGAGCTTGGGAATCCTGTGTG
Band lengths:
243-382
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]