DreEX6078217 @ danRer10
Exon Skipping
Gene
ENSDARG00000032079 | acsl3a
Description
acyl-CoA synthetase long-chain family member 3a [Source:ZFIN;Acc:ZDB-GENE-050420-181]
Coordinates
chr15:43058595-43098446:+
Coord C1 exon
chr15:43058595-43058772
Coord A exon
chr15:43095538-43095647
Coord C2 exon
chr15:43098308-43098446
Length
110 bp
Sequences
Splice sites
3' ss Seq
GTTACGTTTGTGTTTTCCAGTGG
3' ss Score
7.75
5' ss Seq
AAGGTGATC
5' ss Score
4.41
Exon sequences
Seq C1 exon
GTGATTCTGGGAGAGTATCGCTGGATGTCATATGAGCAGGTTTTTAAAGAAGTGAATGCTTTTGGAAGCGGTTTGGCTGCTCTTGGACAGAAACCAATGAAAAACATTGCCATCTTCTGCGAGACGAGAGCTGAGTGGATCATAGCAGCGCAGGCCTGCTTCATGTACAACTTCCCAC
Seq A exon
TGGTAACACTTTACTCTACTCTGGGTGGTCCTGCTATTGCTCATGGGCTCAATGAAGCCGAAGTTTCTCACATCATCACCAGCAAGGATCTTCTGGAAACCAAACTCAAG
Seq C2 exon
AGCATTTTCATCGAGGTGCCCAGATTGTGCCACGTCATCGTGGTTGACGATAAGCTCAGCACCAGCTCTGAATACCCACGAGGCATCAGCATCCACAGCATGGCGTCGGTGAAAGAACTTGGGGCTCGTCCTGAGAATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000032079-'5-5,'5-4,6-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.128
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(12.4=100)
A:
PF0050123=AMP-binding=FE(7.6=100)
C2:
PF0050123=AMP-binding=FE(9.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTTTTGGAAGCGGTTTGGCTG
R:
ACGAGCCCCAAGTTCTTTCAC
Band lengths:
249-359
Functional annotations
There are 1 annotated functions for this event
PMID: 20429931
[Inferred from paralogous MUTEX event in ASCL6]. Isoforms with different fatty acid Gate-domain motifs (by inclusion of mutually exclusive exons) have different activity and the form lacking this domain, isoform 3 (including no exon), showed no detectable activity.
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]