DreEX6033893 @ danRer10
Exon Skipping
Gene
ENSDARG00000026489 | khsrp
Description
KH-type splicing regulatory protein [Source:ZFIN;Acc:ZDB-GENE-030131-4357]
Coordinates
chr1:54291692-54294585:-
Coord C1 exon
chr1:54294370-54294585
Coord A exon
chr1:54291831-54291936
Coord C2 exon
chr1:54291692-54291745
Length
106 bp
Sequences
Splice sites
3' ss Seq
TTTATGTCTCTGTTGTCCAGATT
3' ss Score
7.03
5' ss Seq
GAGGTATGT
5' ss Score
9.81
Exon sequences
Seq C1 exon
TTTTGTCCCTCCCATGCGTTTGTCTCGCTTGTCTTCCCGCGTCTGCGCACCTCCGCCCGGCCTAGACCTCCCCGCCGTATCCCGCACACTTCACCCTTCCGCACGGACAGCATGTCTGAGTACAGCGCGGTGCCGCCGCCCGGGCCCGGGGCGGCTTTAGGAGCCGGTGGGCTGAAAAAAGACGCGTTCGCGGACGCGGTACAGCGCGCGCGGCAG
Seq A exon
ATTGCAGCAAAGATCGGTGGTGACGTTACAGGTCCCCCAATGAGCAATAACGGCGGAGCGGAGAGTTATCCCTTCCCGACGCCGAAGCGATCGCTGGAGGATGGAG
Seq C2 exon
ATCAGCCTGAAAGCAAGAAGATGGCGTCTGACAGGGACAACGCTGCAGCTCTAT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000026489-'0-2,'0-1,3-2=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.629 A=0.981 C2=0.842
Domain overlap (PFAM):
C1:
PF044029=SIMPL=PU(21.8=48.6)
A:
PF044029=SIMPL=FE(44.9=100)
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TTTTGTCCCTCCCATGCGTTT
R:
TGTCAGACGCCATCTTCTTGC
Band lengths:
249-355
Functional annotations
There are 1 annotated functions for this event
PMID: 16914741
Partially encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: glutathione s tranferase tag, mutation analysis, pull down, two hybrid. ELM ID: ELMI002513; ELM sequence: FADAVQRARQI; Overlap: PARTIAL_LEFT
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]