DreEX6034087 @ danRer10
Exon Skipping
Gene
ENSDARG00000053462 | c3a.5
Description
complement component c3a, duplicate 5 [Source:ZFIN;Acc:ZDB-GENE-090311-30]
Coordinates
chr1:55447940-55449420:-
Coord C1 exon
chr1:55449305-55449420
Coord A exon
chr1:55449076-55449222
Coord C2 exon
chr1:55447940-55448140
Length
147 bp
Sequences
Splice sites
3' ss Seq
GTTTTTCGTTATGTCTTTAGGTC
3' ss Score
9.89
5' ss Seq
ACTGTAAGA
5' ss Score
4.8
Exon sequences
Seq C1 exon
GCAGTGAAATGGTGGATGCGGAAAAGAAAGGCATTCAGATTGTGACGTCTCCATACTCGATCTATTTCAAACGAACACCGCAATTCTTCAAACCCGGGATGTCCTTCGATGTCTCG
Seq A exon
GTCTACGTAACAAATCCTGATGGGACACCTGCTGAAAATGTGGAGGTGGAGGTCAATCCTGGAGGGGTCAAAGGTCGGACAAAACCCAACGGCATCACTAAAGTGACCATCAACACATTGGGAGGATCTTCAACATTGGAGATCACT
Seq C2 exon
GCGAATACCAAAGATCCGCAGTTAAGCACTGACCAGCAGGCAGTGAAGAAGATGACTGCTCGGGCCTACAAACCTAAAGGCGCCAACAATTACCTGCACATCGGCATCGATGCCGCAGAGCTCGAGATCGGGGATCAGATGAAGGTCAACCTGAATCTGGGAAATGGTGCTGGAGTGGTGAACCAGGATTTCACATATATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000053462-'8-9,'8-8,9-9=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.025 A=0.520 C2=0.279
Domain overlap (PFAM):
C1:
NO
A:
NO
C2:
PF077039=A2M_N_2=PU(24.8=51.5)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CAGTGAAATGGTGGATGCGGA
R:
ATCTGATCCCCGATCTCGAGC
Band lengths:
255-402
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]