DreEX6040362 @ danRer10
Exon Skipping
Gene
ENSDARG00000061904 | fhod3b
Description
formin homology 2 domain containing 3b [Source:ZFIN;Acc:ZDB-GENE-081028-61]
Coordinates
chr16:18889193-18891331:-
Coord C1 exon
chr16:18891220-18891331
Coord A exon
chr16:18889436-18889530
Coord C2 exon
chr16:18889193-18889336
Length
95 bp
Sequences
Splice sites
3' ss Seq
TTGCTTTTATGCATGTCCAGGCT
3' ss Score
5.43
5' ss Seq
AAGGTACTT
5' ss Score
8.4
Exon sequences
Seq C1 exon
TTCCGGTTGGTTGTGAAAACTGCTATGAAGCTGCTGCTGGTTTTTGTGGAGTACACCGAGAGCAACGCACCTCTCCTCATCCAAGCCGTCAACAGCGTTGACACCAAACAAG
Seq A exon
GCTCTAAGCCCTGGTCTAATGCCATGGAAATCCTTGACGAGAAAGATGGAATGGACACTGAGCTGCTGGTTTATGCCATGACACTAATCAACAAG
Seq C2 exon
ACATTAGCTGGTCTGCCTGACCAGGACTCCTTCTATGATGTGGTTGACAGTCTGGAGGAGCAGAACATGGAGGCTGTTGCCCAAAGACACATTAGTAGAAAGGGCACAGATCTGGACTTGGTAGAGCAGTTCAACATTTATGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000061904-'10-12,'10-11,11-12=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.083 C2=0.146
Domain overlap (PFAM):
C1:
PF063718=Drf_GBD=PD(10.4=39.5)
A:
NO
C2:
NO
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Chicken
(galGal3)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CCGGTTGGTTGTGAAAACTGC
R:
TGTTCTGCTCCTCCAGACTGT
Band lengths:
177-272
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]