DreEX6065820 @ danRer10
Exon Skipping
Gene
ENSDARG00000060841 | pik3c2a
Description
phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha [Source:ZFIN;Acc:ZDB-GENE-030328-39]
Coordinates
chr18:27081288-27082030:+
Coord C1 exon
chr18:27081288-27081457
Coord A exon
chr18:27081546-27081702
Coord C2 exon
chr18:27081921-27082030
Length
157 bp
Sequences
Splice sites
3' ss Seq
ATCGTAATTGTTTCCTATAGGGA
3' ss Score
7.56
5' ss Seq
CTGGTAATA
5' ss Score
4.88
Exon sequences
Seq C1 exon
GCGTCTGAGAATTTTATCTACACCTGTGCTGGATGCTGTGTGGCCACTTACATCCTCGGCATCTGCGATCGTCACAACGACAACATCATGCTGCGCTCCACTGGCCACATGTTCCACATAGACTTCGGGAAGTTCCTGGGTCACGCACAAATGATTGGGAGTTTTAAAAG
Seq A exon
GGACCGAGCACCGTTTGTCTTAACCTCTGACATGGCTTATGTCATCAATGGAGGCGAAAGACCCACTAGTCGCTTTCAGCTGTTCGTTGACCTTTGCTCGCAAGCCTACAACCTGATCCGCAAACACTCCAACCTCTTCCTCAACCTGCTATCTCTG
Seq C2 exon
ATGACACAATCAGGTTTACCCGAGCTGACCGGAGTCCAAGATTTGAAGTATGTGTATGATGCTCTTCAGCCTCAGACTACAGATGCAGAGGCTACCATTTTCTTCACAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000060841-'29-32,'29-31,30-32=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0045422=PI3_PI4_kinase=FE(25.9=100)
A:
PF0045422=PI3_PI4_kinase=FE(24.1=100)
C2:
PF0045422=PI3_PI4_kinase=PD(4.6=27.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTACACCTGTGCTGGATGCTG
R:
TGGTAGCCTCTGCATCTGTAGT
Band lengths:
250-407
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]