MmuEX0035117 @ mm9
Exon Skipping
Gene
ENSMUSG00000030660 | Pik3c2a
Description
phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide [Source:MGI Symbol;Acc:MGI:1203729]
Coordinates
chr7:123493547-123495481:-
Coord C1 exon
chr7:123495312-123495481
Coord A exon
chr7:123494287-123494443
Coord C2 exon
chr7:123493547-123493656
Length
157 bp
Sequences
Splice sites
3' ss Seq
CCTCTGTGTACTTTCAACAGGGA
3' ss Score
9.34
5' ss Seq
CTGGTAACC
5' ss Score
4.51
Exon sequences
Seq C1 exon
GCTTCTGAGAACTTTATCTACTCTTGTGCTGGGTGCTGTGTAGCCACCTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGAAGCACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCAAAAG
Seq A exon
GGACCGAGCTCCTTTTGTGCTTACCTCTGACATGGCGTATGTCATTAATGGAGGTGAAAAGCCCACCATTCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTCTTTCTTAACCTTCTCTCACTG
Seq C2 exon
ATGATTCCTTCAGGATTGCCAGAACTCACAAGTATTCAGGATCTGAAATATGTTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSMUSG00000030660_MULTIEX1-2/2=1-C2
Average complexity
S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0045422=PI3_PI4_kinase=FE(25.9=100)
A:
PF0045422=PI3_PI4_kinase=FE(24.1=100)
C2:
PF0045422=PI3_PI4_kinase=PD(4.6=27.0)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Other assemblies
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CTACTCTTGTGCTGGGTGCTG
R:
TCAGCATCTGTAGTTTGGGGC
Band lengths:
242-399
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]
SPECIAL DATASETS
- Pre-implantation embryo development
- Neural differentiation time course
- Muscular differentiation time course
- Spermatogenesis cell types
- Reprogramming of fibroblasts to iPSCs
- Hematopoietic precursors and cell types
Other AS DBs: