DreEX6071489 @ danRer10
Exon Skipping
Gene
ENSDARG00000075271 | rapgef5a
Description
Rap guanine nucleotide exchange factor (GEF) 5a [Source:ZFIN;Acc:ZDB-GENE-030131-7681]
Coordinates
chr19:2856406-2857445:+
Coord C1 exon
chr19:2856406-2856493
Coord A exon
chr19:2857254-2857318
Coord C2 exon
chr19:2857391-2857445
Length
65 bp
Sequences
Splice sites
3' ss Seq
TGATTTCTGCTGTTTTTCAGACA
3' ss Score
8.8
5' ss Seq
CTGGTGAGT
5' ss Score
10.1
Exon sequences
Seq C1 exon
GATCCATCCATGAACCACAAAGCCTACAGAGACGCTTTCAAGAAAATGAAGCCGCCCAAAATACCCTTCATGCCGCTGCTGCTCAAAG
Seq A exon
ACATCACCTTCATCCACGAGGGGAACAAAACATTTCACGATAACCTGGTGAACTTTGAGAAGCTG
Seq C2 exon
CACATGATCGCAGACACGGTCCGACTCATTCGCCACTGTCAGATGGATCAGACAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000075271-'14-15,'14-14,15-15=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.033 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0061714=RasGEF=FE(15.7=100)
A:
PF0061714=RasGEF=FE(11.4=100)
C2:
PF0061714=RasGEF=PD(0.5=5.3)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
TCCATCCATGAACCACAAAGCC
R:
CTGATCCATCTGACAGTGGCG
Band lengths:
137-202
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]