DreEX6079188 @ danRer10
Exon Skipping
Gene
ENSDARG00000058543 | lama5
Description
laminin, alpha 5 [Source:ZFIN;Acc:ZDB-GENE-030131-9823]
Coordinates
chr23:9696527-9701064:-
Coord C1 exon
chr23:9700863-9701064
Coord A exon
chr23:9696766-9696910
Coord C2 exon
chr23:9696527-9696667
Length
145 bp
Sequences
Splice sites
3' ss Seq
TTACATCACTCTCCCCTCAGGTG
3' ss Score
11.37
5' ss Seq
AAGGTGTCT
5' ss Score
2.57
Exon sequences
Seq C1 exon
CATGTAACTGTGGTTCGCGTCTGTGTGAGCCTGTGACGGGCGAATGCATTTGTCCACCACGGACCCTTCAGCCAGACTGTGTAACCTGCGAGCCCCAGACGTTTGGCTGCCATCCTCTGGTGGGCTGTGAGATGTGTAACTGCTCCAGGCCTGGAGTTACGTCGATGGACATCAGTTGTGACACAAACAACGGGCAATGCAG
Seq A exon
GTGCAGGAACAACATTGTTGGCCGTCAGTGTGACAGGTGTTCTCCAGGTTTCTATGGTTACCCCAACTGCAGGCCATGTAACTGCAATGAGGCGGGAACTGAGATGAATGTTTGCGACTCCTTCACTGGACGCTGTCTGTGCAAG
Seq C2 exon
GAAAATGTGGAAGGCCCTCGCTGTGACCAGTGCAAACTGGGAACGTTTCATCTGGACCCCACTAATGCTAAAGGCTGCACCAAGTGCTTTTGCTTTGGAGCCACTGATCGCTGCCACAGCTCTGACAAACGCCGAAGTGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058543-'39-36,'39-35,40-36=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0005319=Laminin_EGF=PD(6.1=4.4),PF0005319=Laminin_EGF=PU(45.8=32.4)
A:
PF0005319=Laminin_EGF=PD(52.1=51.0),PF0005319=Laminin_EGF=PU(44.9=44.9)
C2:
PF0005319=Laminin_EGF=PD(51.0=53.2)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GTAACTGTGGTTCGCGTCTGT
R:
GGGTCCAGATGAAACGTTCCC
Band lengths:
258-403
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]