DreEX6080171 @ danRer10
Exon Skipping
Gene
ENSDARG00000079199 | megf6a
Description
multiple EGF-like-domains 6a [Source:ZFIN;Acc:ZDB-GENE-090312-12]
Coordinates
chr23:21218022-21219928:-
Coord C1 exon
chr23:21219797-21219928
Coord A exon
chr23:21219451-21219579
Coord C2 exon
chr23:21218022-21218153
Length
129 bp
Sequences
Splice sites
3' ss Seq
TTCTGTTCTATATCTCTTAGTCT
3' ss Score
7.26
5' ss Seq
AAGGTAAGG
5' ss Score
10.51
Exon sequences
Seq C1 exon
AGTGTGAAGAGGGCCGTTGGGGTCTTGGCTGTGCAGAATCATGTCCTTCCTGTGACAATGGTGGAGTATGTGATAGACAAAACGGCACCTGTGTTTGTTCGCCGGGATTCATGGGAAATCTCTGTCAGAATG
Seq A exon
TCTGTCCAGATGGGCACTTTGGCCTGAGCTGCCTGTTGCGCTGCTCCTGTCAAAACGAAGGGAAATGCCACCATGTGACTGGCCTCTGTGTTTGTAAAGATGGCTGGATGGGACCCAACTGCGCAAAAG
Seq C2 exon
CTTGTGTGGCTGGACGATGGGGGCCGAACTGTGCCAACCAGTGTGACTGCAGGAGCAGCGTGGGCAGCTGTGACCCTGTGATTGGCCTGTGTCATTGTGAAGCGGGCTACATGGGACCACGCTGTGATCAAA
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000079199-'20-17,'20-16,21-17=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
Alternative protein isoforms (Ref)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF079748=EGF_2=WD(100=71.1)
A:
PF079748=EGF_2=WD(100=61.4)
C2:
PF0005319=Laminin_EGF=PU(64.6=68.9)
Main Skipping Isoform:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTCTTGGCTGTGCAGAATCA
R:
TTGATCACAGCGTGGTCCCAT
Band lengths:
243-372
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]