DreEX6083161 @ danRer10
Exon Skipping
Gene
ENSDARG00000023362 | nr5a1b
Description
nuclear receptor subfamily 5, group A, member 1b [Source:ZFIN;Acc:ZDB-GENE-040702-6]
Coordinates
chr21:8185770-8220263:+
Coord C1 exon
chr21:8185770-8185911
Coord A exon
chr21:8190882-8191624
Coord C2 exon
chr21:8220144-8220263
Length
743 bp
Sequences
Splice sites
3' ss Seq
ATCATTGTTTGTGCTCACAGCTG
3' ss Score
7.9
5' ss Seq
AAGGTTAGT
5' ss Score
8.54
Exon sequences
Seq C1 exon
GGTTTCTTTAAAAGGACCGTACAGAATAACAAACGGTACACATGTGCAGAGAGTCAGGACTGTAAGATTGATAAAACCCAGAGGAAACGGTGTCCTTTCTGCCGCTTCCAGAAATGTCTCAACGTCGGGATGCGACTGGAAG
Seq A exon
CTGTCCGTGCGGATAGGATGCGAGGAGGCCGCAACAAATTCGGCCCCATGTATAAGCGAGACCGGGCGTTAAAGCAGCAGAAGAAAGCTCTCATCCGGGCCAGTGGCTTAAAGATGGAAGCCACACCCCCTTTGCTGACGTCACCACAACCTGACTACACCTTCAGCACAGCTTTGTCTGTGCCAGCTCCGAAAAACACCCATCCGAACATCGTCACCTCTGTGGCACCCACCGATTACGAACGCAGCCTCTACGCATCCAGCTCCCTGAGTTTATCCGTCCCCATCCCGGCCCACACTCCTCTCCCAGCACAATACCCCTATCCAAATCTGCCCAGCCGTGCCATAAAGTCTGAGTATCCTGACCACTACACAAGCTCAGAGCATTACACCAGTGCCAGCTCCCCAGAGTCAGTGCCAGGCTACACGTATATCGAGCAAACACGGGTGTCCAGCAGCCCGCAGGTGGTGGCACCGGGTTTAACAGTGCCGCCTTTGGTTCTGGAGTTTGTTCGCTGTGAGCAGGATGAGCTCCAGGTGCAAAGCAAAATAAGCGCTCACCTGGCGCACCTGCAGCAGGAGCAGAACTCACGGAGCACGGCAGCCAATCAGGAGCAGAGCTCCCGCCTCGCAGCCAAACCCGAGCGGCTCAGCACATTTGGCCTCATGTGCCACATGGCCGACCAGACGCTCTTCTCTATCGTGGAGTGGGCACGAAGCTGCATCTTCTTTAAGGAGCTTAAG
Seq C2 exon
GTTGGAGATCAGATGAAGTTGCTGCATAACTGCTGGAGTGAACTTCTAGTGCTGGACTATGTTGCCAGACAGTTGCATCATGGGAAAGAGGACAGTGTGCTTCTCATTACTGGACAGGAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000023362-'2-3,'2-2,3-3=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.351 C2=0.000
Domain overlap (PFAM):
C1:
PF0010513=zf-C4=PD(64.3=93.8)
A:
PF0010425=Hormone_recep=PU(22.6=19.4)
C2:
PF0010425=Hormone_recep=FE(18.4=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Rat
(rn6)
No conservation detected
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGTTTCTTTAAAAGGACCGTACAGA
R:
CTCCTGTCCAGTAATGAGAAGCAC
Band lengths:
262-1005
Functional annotations
There are 1 annotated functions for this event
PMID: 19995909
Encodes an experimentally validated Eukaryotic Linear Motif (ELM). Method: coimmunoprecipitation, gal4 vp16 complementation, mutation analysis. ELM ID: ELMI002003; ELM sequence: PYASPP; Overlap: FULL
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]