DreEX6098298 @ danRer10
Exon Skipping
Gene
ENSDARG00000042667 | klf2a
Description
Kruppel-like factor 2a [Source:ZFIN;Acc:ZDB-GENE-011109-1]
Coordinates
chr22:15871951-15874649:+
Coord C1 exon
chr22:15871951-15872341
Coord A exon
chr22:15872756-15873650
Coord C2 exon
chr22:15873756-15874649
Length
895 bp
Sequences
Splice sites
3' ss Seq
ATCAACCTGTTTCATTGCAGAGG
3' ss Score
8.39
5' ss Seq
CAGGTAAAT
5' ss Score
8.76
Exon sequences
Seq C1 exon
GTGATTTAAAGAAGAGCCCGTGCGCTCTCAACATCCAAGACGAAAGGCGCACACGCCGGAGAGCAGCAGCTATATACCGGGGCTAAAATTAGGGACGGATAGTGCGTATTTCCTCCGCTTCTTATAATTGGTCCAGCTTGCGCTGCCGTGATATTTATAAGATAAAGGTCTTCCACCACTCATACTTTCTGCATGAGTGTTAAACATAGTGTAAAGAGCAGAGCAGAGTTGTAGTGCACTACAACCAAAGTTTCTCCGAGGGATTGCAACAAATCCCCGATCTTTAAAGTTTGTTTTACCTTAACTGGGACGACTGGAAATGGCTTTGAGTGGAACGATTTTACCGTCTATTTCCACATTTTCGGCGCAGAAGGAAAAATGCTGGGAGAAC
Seq A exon
AGGTGGAAGGATGAACTGGACAGGTCCATGCATCTCAGCTGCATAGCTGACCTATCTAACATGGACAACCTCAACAGAATAACAGACGACGAAGATCTTGACAAGTACCTGGATCTGGAGTTTATACTGGCCAACACTGCAGGTTCTGACAATCTCGGACTCGGCATGGCAGGCGACTACAGAATGCAAGAGTCCAGAAACATGTACAACCCGACTACATACTCGGTGCCTGAGATCAACCCATCACCACCTCCACCGTACACCACCAGCCTCATGGCAGAGCTTCTGCAGTCTGACATTGACACCTACTGCCAACCGTCTCTCCAGGGAAGATTCCTAGTCAGGCCTGCTTTCCCCACTCAGGACATTTCGGAGTGTATCAAAGTGGAGCCCTGCATGGATAGTTACGGACCTGTACGAGGAATGGTTCCCAAAGTCAAACAAGAGGGCAACGGTGCATGCATGAGATCCTATGAGCAGCCAAGACTAGCCAACTCTCCCCAGGGAGCAGGCAGCATGACTCCCCCACAGAGCCCTGCGGAGCTCATCAACACAGACTGTCAGTCACACTCGCAGATGTGCCATGCCTTAACGTTCAGCCAGAGCTATCAAGGCAACACAGGATTTCCCCACGCAGCACCTCCACAGATGCAGCTGCCTTACCAAAGCACACATCACTTCAGCATGTGCGACGATGGGCTTGCAATGCCCAACGCCAACCAAAGAGTACTTCTGACACCACCCTCATCTCCTCTAGAGCTGGACGCCAAACCAAAGAGGGGGCGCCGCACGTGGCCGAGGAAACGCACGGCGACTCACACTTGCACTTTTTCTGGATGTGGCAAAACTTACACAAAAAGTTCTCACTTGAAGGCTCATCACAGAACACACACAG
Seq C2 exon
GCGAGAAGCCCTATCATTGCAGTTGGGAAGGCTGTGGGTGGAAGTTCGCCCGTTCTGATGAATTGACCCGTCACTTCCGCAAGCACACCGGACACCGACCCTTCCAGTGCCACCTGTGCGAACGTGCCTTCTCCAGGTCCGACCATCTCGCCCTTCATATGAAACGTCATATGTAGGAACGGACTCTGGAAAATGTGGACTAAACGCACTGAACAGTGAACATTGTCTGTACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCTTATTTAAAGTATGGTGAAGGGGAAACATGAACTGACTTGACCTATTGCTTGTAGCTGGTTCTACACTTCAATTCTTTACAGAATTTACAGAAACAAACTATATTTTTCGTTTCCAAAGAGCAAGTTCAACTCATACTATAGGCTGGACCTAAACCTCAAGACGGAACTGGAATCAGACAGTATAATGTGCTTTAAGTTAACATGATGTGCTGCGGACATCACGACAGTGCCT
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000042667-'0-1,'0-0,1-1=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.421 C2=0.103
Domain overlap (PFAM):
C1:
NO
A:
PF134651=zf-H2C2_2=PU(35.7=3.3)
C2:
PF134651=zf-H2C2_2=PD(60.7=28.8),PF134651=zf-H2C2_2=WD(100=44.1)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
CGGGGCTAAAATTAGGGACGG
R:
CAGCCTTCCCAACTGCAATGA
Band lengths:
348-1243
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]