DreEX6102137 @ danRer10
Exon Skipping
Gene
ENSDARG00000045803 | mfge8b
Description
milk fat globule-EGF factor 8 protein b [Source:ZFIN;Acc:ZDB-GENE-070209-137]
Coordinates
chr25:19077643-19081217:+
Coord C1 exon
chr25:19077643-19077756
Coord A exon
chr25:19080218-19080399
Coord C2 exon
chr25:19081062-19081217
Length
182 bp
Sequences
Splice sites
3' ss Seq
TGTCTGCTTCTTTTTCCCAGGAT
3' ss Score
9.94
5' ss Seq
GAGGTAAAC
5' ss Score
7.2
Exon sequences
Seq C1 exon
ATGTGAATGACTGTGCGGGTCAGCCCTGTCTAAACGGAGGCACCTGTCAAGATCTTGATGGGGACTTCAACTGTAAATGTCCCTCGCCCTATGTTGGAAAACACTGCAATCTGC
Seq A exon
GATGCATCTCTCTGCTGGGAATGGAAGGAGGAGGAATTGCTGAGTCTCAGATCTCTGCATCTTCAGTGTACTATGGCATTCTGGGACTGCAGCGATGGGGACCCGAGTTGGCTCGGCTTAATAACAAGGGACTTGTTAATGCTTGGACCTCTGCAACGCATGACAAAAACCCCTGGATTGAG
Seq C2 exon
GTCAACCTGCAGAGGAAGATGCGCTTCACTGGCATCATTACCCAGGGTGCCAGTCGCATGGGCACAGCCGAGTTCATCAAAGCCTTCAAGGTGGCGTCCAGTTTGGATGGAAGAACCTACACCATGTACAGACCTGAGGGCCAGAGCAAAGATATG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000045803-'4-5,'4-4,5-5=AN
Average complexity
A_S
Mappability confidence:
100%=100=100%
Protein Impact
ORF disruption upon sequence exclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=0.000 C2=0.000
Domain overlap (PFAM):
C1:
PF0000822=EGF=PD(89.5=87.2)
A:
PF0075420=F5_F8_type_C=PU(31.7=72.1)
C2:
PF0075420=F5_F8_type_C=FE(36.7=100)
Main Skipping Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
ATGTGAATGACTGTGCGGGTC
R:
CTGGCCCTCAGGTCTGTACAT
Band lengths:
258-440
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]