DreINT0009481 @ danRer10
Intron Retention
Gene
ENSDARG00000073731 | DOK4
Description
docking protein 4 [Source:HGNC Symbol;Acc:HGNC:19868]
Coordinates
chr7:28715556-28716276:+
Coord C1 exon
chr7:28715556-28715745
Coord A exon
chr7:28715746-28716137
Coord C2 exon
chr7:28716138-28716276
Length
392 bp
Sequences
Splice sites
5' ss Seq
CAGGTATAA
5' ss Score
7.46
3' ss Seq
TAATCTTGCTTTTTTTTTAGGAT
3' ss Score
10.26
Exon sequences
Seq C1 exon
AGCGTTTTAATGTGTTCCTGTTGCCCTGCCCAAATCTGGACATCTACGGTGAGTGTATGATGCAGATCACACATGAAAACATCTACCTGTGGGACATTCACAACCCTCGCACCAAGCTGGTAACCTGGCCGCTTTGTTCGCTACGCCGATACGGACGAGACGCTACACGCTTCACCTTCGAGGCTGGCAG
Seq A exon
GTATAAAAATAATCAAAACACCATTAAAAATTGAAAGACAATTCACAGGAACTTGTAAAATGTGAATCTCAGTCTGATGTGGTCATGTAGGGAAAGCATGGAGGAAGCTATTGAACCATGGTGGACCTCCTAAAAGGGTTTATTGAGTGGATTACAGGAAGTTCAGTGGCAGATTAGGTGTTCTTATCTGTCTCTTTGCTTTGATTAAATAATAAAAGACGATTCACATCACCAGCTGGATGCCCTTTGTTGGAAAAGATGAGAGAAAATTCGGCGTTTGATATCGCACTGACCCCTAGTGGCTGTTAATATGAATCACACAAATGTTTAGCGTGTATGCGTTTTTTCTATGCTGAATTGAGTATTGAGTGTTAATCTTGCTTTTTTTTTAG
Seq C2 exon
GATGTGTGACAGTGGGGAGGGCCTGTACACTTTCCAGACACGGGAAGGAGAGCAGATCTACCAAAGGGTCCATTCTTCCACGCTGGCTATAGCTGAGCAGCATAAGAGAGTCCTCATGGAAATGGAGAAAAACAGCAAG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000073731:ENSDART00000113998:6
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.106
Domain overlap (PFAM):
C1:
PF0217412=IRS=FE(64.3=100)
A:
NA
C2:
PF0217412=IRS=PD(32.7=68.1)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCGTTTTAATGTGTTCCTGTTGC
R:
GCTGTTTTTCTCCATTTCCATGAGG
Band lengths:
326-718
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]