DreINT0018568 @ danRer10
Intron Retention
Gene
ENSDARG00000058944 | SBK2
Description
SH3 domain binding kinase family, member 2 [Source:HGNC Symbol;Acc:HGNC:34416]
Coordinates
chr8:10004373-10006475:-
Coord C1 exon
chr8:10006290-10006475
Coord A exon
chr8:10004576-10006289
Coord C2 exon
chr8:10004373-10004575
Length
1714 bp
Sequences
Splice sites
5' ss Seq
AAGGTACAG
5' ss Score
8.04
3' ss Seq
TTTTTTGTTTTTTTAACAAGGCA
3' ss Score
6.53
Exon sequences
Seq C1 exon
TCAGAACAGGCGGTGTGATGATTGAGCTGGGCTCAGTGGAGGATGGCAGCAGTCTGATAGACGAGCTGATGGAATTGACGTCTCAGAGTTTATGTAAGCTCGAGATTCCAGAGCATTTCAACATCATTAAAGAGATTGGCCGTGGCAAGTATGGCCGTGTGCTGCTGGTCACCCACAGATGCAAAG
Seq A exon
GTACAGTATATTCAATAGATTACTGAAAGAACTTTACACGCATATCTGCAAGATTTCACAAATTTGGTCAAAATCCTGTTTAGCAAATAAAGAATGGCGTCCATTGACTCCATTCCAAAGTCATTGGTAAAAATATAACTGAAATCTCAATCTCTATGAGTAACAACAACAATAGCGATATTACTAATACAAAAATATTACATAACAATTTTATTATTGTTGTTGTTGCTGGTTTGCTGTACTAATAAGTGTAATTATGTTTCTTTTTAATTATTCATTCATTCATTCCCCTTTGACTTAGTGTCTAATTTATCAGGGGTCACCACAACGGATTGAACCGCCAACTACTCTGGCATGTTTTACAGTGCGCAGCGGATGGCCTTTTAGCCACAACCCAGCACTTTACTGTCATTCAAAATATACTGGTGTACAGAGAAAGAAATTTTGTTGCAATGGCTCAGCATGAAAAAAAAAACACTAAACAATAGATGAAAAATAACAATGTGCAAAGCACACATTATTATTACTGAATGAGCAATTGACTGAAATACCCCCATAAAAGAATTTCAATTACAAATGAATGAGATGAAAATAAAAGACATGCCTCAATGCCTTCAAGAGTTTCCCGCCAGAGAAAATGACATCACTTGGAGCAAGTCATATTCTATCAGTGCTTTCAAGATTGTTACGCCATATTATAATCAATGTTAACTAGAATGTGACTGAAATTTTTACTGAAATGACAAATAATATAGAAAATATATCGGAGAGGCAACAAATCAGCTTTCTTAGCCATTGAACTGAACATGCCGAGCTCATGTAAATACTGTAGTACATAAACATGAGTATTATTCTCTAGAACGGCTTGTGTGTGTGTATGATGTTCTGCCAAACATATCTGCTGTACTGAACACTTTGACCAGAGCAGAATGAGATAATGCTCTTTAAATGAAACACTAGCCCCTCTTTTACACTCCCACAGATCGCAATCCCAAGATATCATCCCAACAGCTGTGGAAAAACTTTCTATTAACAACTTAAAGACTGTATAGAGCCTGCATCATTCTTCTCATACAGTGAGGGGAAATAAGTATTGAACATGTCAGCATTTCTCTCAGAAAACATATTTTTAAAGGTGCTGACTTGAAATTTGCACGGGATGTTGGTAACAACCAAAGCAACCCATATATGCAAAGAAAACAAATCAAATTCGTTCACAAAACGACACAGGGAAAAAGTGTTGAACACATGAAAAAAGGGAGGTGTAGAAAGGCCAGACAGCAAATGAAATCTCTCAGTAGTTCTTCAGCAACTCTTTGCCCATCATTGCATATTAACATTAGATCCTTCAGTAAAACATCTACATCAGGATGATGAATATGAAACCAGGGTGGACATTATAGCAAGAAAAAAAAATCCACAACACAGCCAAGGAAACTCTTAAATGGTTTCAGAGAAAGAAAATAAAGCTGTAGAATGGCCCAACCAATCACCTGACTTAAAGCTAAAGATCAGATATGATAGATGAGACCCACAGAACCATCAAGATTTTTACACTCTGTTTAAGTCTGTGAAAACAATGCATGTGACTTCAAAAACTTGATGCTTTCAATACTTATTTCCCCCACTGTACTGTATCTAGAGCAGCTTTAGACGGATAAAGTCTTGTTTGTTGTTTCTTGTTTGTTTTTGTTTTTTTTGTTTTTTTAACAAG
Seq C2 exon
GCACTCCAATGGCTCTGAAAGTGCTCCCGAAGGCCTCCACCAAACTGCAGGGATTTCTCAGAGAATATTGCATCTCTCTGCATCTGTCCTGTCATCCCTGCATTGTAGGATTGTTTGGCATTGCCTTCCAGTCCAACGAGCACTACGGATTCGCCCAGGAGCTCGTCACAGGACGGGATCTGTTTGCCATCATTCAGCCACGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000058944:ENSDART00000138411:2
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion (1st CDS intron)
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.000
Domain overlap (PFAM):
C1:
PF0006920=Pkinase=PU(8.5=40.4)
A:
NA
C2:
PF0006920=Pkinase=FE(25.2=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
GGAGGATGGCAGCAGTCTGAT
R:
CGTGGCTGAATGATGGCAAAC
Band lengths:
351-2065
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]