DreINT0025526 @ danRer10
Intron Retention
Gene
ENSDARG00000004078 | acsl4a
Description
acyl-CoA synthetase long-chain family member 4a [Source:ZFIN;Acc:ZDB-GENE-040426-1565]
Coordinates
chr14:28140381-28140857:+
Coord C1 exon
chr14:28140381-28140519
Coord A exon
chr14:28140520-28140706
Coord C2 exon
chr14:28140707-28140857
Length
187 bp
Sequences
Splice sites
5' ss Seq
ACTGTAAGT
5' ss Score
8.59
3' ss Seq
ACAATTGTTTTGTTTTTCAGTGA
3' ss Score
9.25
Exon sequences
Seq C1 exon
AGTGTTCTTCTTCAAATTCCCAATTTAAAGCACATCATCTACGTGGACAATAAGAGCATGAACACAGCAGGATTTCCACAGGGCATCCAGATCCACAGCATGCACTCAGTTGAAGAGCTGGGAGCCAAGCCAGAAAACT
Seq A exon
GTAAGTACCGTTAAGTCTTTAAAAGCACCAACTTCACAGTTGACATTTTATTTATATGCTAATTAACATTCTCTCTAGTTTATCCAATGTTCAACCACCACACAATATTGATTTGAATCAAAATTTGCACTGTAATGGATTCGTCTCCATCAGCAGAGAAATAAACAACAATTGTTTTGTTTTTCAG
Seq C2 exon
TGAATAGACCAATAGTGAAGCCCGTCCCATCAGATCTTGCTGTTATTATGTACACCAGCGGCTCCACCGGGAGACCTAAAGGGGTGATGATCATCCACAGTAACCTGATTGGAGGCATGGCAGGCCAGTGTGAGAGGATACCTGGGTTGGG
VastDB Features
Vast-tools module Information
Secondary ID
ENSDARG00000004078:ENSDART00000006489:5
Average complexity
IR
Mappability confidence:
NA
Protein Impact
ORF disruption upon sequence inclusion
No structure available
Features
Disorder rate (Iupred):
C1=0.000 A=NA C2=0.020
Domain overlap (PFAM):
C1:
PF0050123=AMP-binding=FE(9.7=100)
A:
NA
C2:
PF0050123=AMP-binding=FE(10.5=100)
Main Inclusion Isoform:
NA
Other Inclusion Isoforms:
NA
Other Skipping Isoforms:
NA
Associated events
Conservation
Fruitfly
(dm6)
No conservation detected
Primers PCR
Suggestions for RT-PCR validation
F:
AGCACATCATCTACGTGGACA
R:
GGTATCCTCTCACACTGGCCT
Band lengths:
253-440
Functional annotations
There are 0 annotated functions for this event
GENOMIC CONTEXT[edit]
INCLUSION PATTERN[edit]